医学信息
醫學信息
의학신식
MEDICAL INFORMATION
2014年
19期
217-218
,共2页
高效液相色谱法%浸煮时间%芍药苷%含量测定
高效液相色譜法%浸煮時間%芍藥苷%含量測定
고효액상색보법%침자시간%작약감%함량측정
High performance liquid chromatography%Soaking time%Paeoniflorin%Content determination
目的研究白芍炮制过程中，浸煮时间对芍药苷含量的影响。方法采用高效液相色谱法测定芍药苷含量，比较不同浸煮时间下，芍药苷含量的变化；色谱条件：Xtimate C18(4.6×250mm,5μm)；流动相：乙腈-0.1%磷酸溶液(14：86)；柱温：30℃；检测波长：230 nm；流速：1 mL/min。结果在20.4~102μg/mL范围内线性关系良好(=0.9993，=5)，平均回收率96.124%，
目的研究白芍砲製過程中，浸煮時間對芍藥苷含量的影響。方法採用高效液相色譜法測定芍藥苷含量，比較不同浸煮時間下，芍藥苷含量的變化；色譜條件：Xtimate C18(4.6×250mm,5μm)；流動相：乙腈-0.1%燐痠溶液(14：86)；柱溫：30℃；檢測波長：230 nm；流速：1 mL/min。結果在20.4~102μg/mL範圍內線性關繫良好(=0.9993，=5)，平均迴收率96.124%，
목적연구백작포제과정중，침자시간대작약감함량적영향。방법채용고효액상색보법측정작약감함량，비교불동침자시간하，작약감함량적변화；색보조건：Xtimate C18(4.6×250mm,5μm)；류동상：을정-0.1%린산용액(14：86)；주온：30℃；검측파장：230 nm；류속：1 mL/min。결과재20.4~102μg/mL범위내선성관계량호(=0.9993，=5)，평균회수솔96.124%，
Objective To establish the dif erent time of steeping and boiling af ect the content of paeoniflorin in White Paeony Root. Methods Use High Performance Liquid Chromatography to determine the content of paeoniflorin, compare with dif erent time of steeping and boiling that the variation of the content of paeoniflorin. chromatographic condition:Xtimate C18 (4.6mmí250mm,5μm); moving phase: acetonitrile-0.1%phosphoric acid (14:86) column temperature: 30℃. determine wavelength:230nm.flow rate:1mL/min. Results There is a good linear relation during 20.4~102μg∕mL (=0.9959,n=5),the average recovery rate 96.124%,RSD=2.29%(n=5);the procedure of White Paeony Root processing,dif erent time of steeping and boiling have a great ef ect on the content of paeoniflorin in White Paeony Root. Conclusion As a result of other composition don't interference with the process ,it is accurate,simple and convenientant.For this reason ,the process can use to determine the content of paeoniflorin .
