医学信息
醫學信息
의학신식
MEDICAL INFORMATION
2014年
19期
48-49
,共2页
邓桂球%苏振宁%张健润%吴茂勇%吴依娜
鄧桂毬%囌振寧%張健潤%吳茂勇%吳依娜
산계구%소진저%장건윤%오무용%오의나
灵芝孢子粉%灵芝多糖%总三萜皂苷%含量测定%紫外分光光度法
靈芝孢子粉%靈芝多糖%總三萜皂苷%含量測定%紫外分光光度法
령지포자분%령지다당%총삼첩조감%함량측정%자외분광광도법
GLS%Polysaccharides%Triterpenoid saponin%Determination%Ultraviolet spectrophotometry
目的建立灵芝孢子粉中灵芝多糖、总三萜皂苷的含量测定方法。方法采用紫外分光光度法，苯酚-浓硫酸比色法于波长492nm处测定灵芝多糖吸光度；以香草醛：高氯酸：冰乙酸(2：8：50)为显色剂，于波长555nm处测定总三萜皂苷的吸光度。结果多糖、总三萜皂苷的标准曲线的线性范围分别为0.0100~0.0300mg(r=0.9982)；0.025~0.125mg(r=0.9996)，多糖、总三萜皂苷的平均加样回收率分别为101.17%、100.74%，RSD分别为1.84%、1.29%。结论该方法简便、快速、准确，可用于灵芝孢子粉中多糖、总三萜皂苷的含量测定。
目的建立靈芝孢子粉中靈芝多糖、總三萜皂苷的含量測定方法。方法採用紫外分光光度法，苯酚-濃硫痠比色法于波長492nm處測定靈芝多糖吸光度；以香草醛：高氯痠：冰乙痠(2：8：50)為顯色劑，于波長555nm處測定總三萜皂苷的吸光度。結果多糖、總三萜皂苷的標準麯線的線性範圍分彆為0.0100~0.0300mg(r=0.9982)；0.025~0.125mg(r=0.9996)，多糖、總三萜皂苷的平均加樣迴收率分彆為101.17%、100.74%，RSD分彆為1.84%、1.29%。結論該方法簡便、快速、準確，可用于靈芝孢子粉中多糖、總三萜皂苷的含量測定。
목적건립령지포자분중령지다당、총삼첩조감적함량측정방법。방법채용자외분광광도법，분분-농류산비색법우파장492nm처측정령지다당흡광도；이향초철：고록산：빙을산(2：8：50)위현색제，우파장555nm처측정총삼첩조감적흡광도。결과다당、총삼첩조감적표준곡선적선성범위분별위0.0100~0.0300mg(r=0.9982)；0.025~0.125mg(r=0.9996)，다당、총삼첩조감적평균가양회수솔분별위101.17%、100.74%，RSD분별위1.84%、1.29%。결론해방법간편、쾌속、준학，가용우령지포자분중다당、총삼첩조감적함량측정。
Objective To establish a method for content determination of total polysaccharides and triterpenoid saponin in the GLS.Methods UV Spectrophotometry,phenol-sulfuric acid;vanillin-glacial acetic acid-sulphuric acid-perchloric acid mixture at the wavelength of 492nm and 555nm absorbance.Results The linear range of total ginseng polysaccharides was 0.0100~0.0300mg(=0.9982);0.025~0.125mg(=0.9996).The recovery rate of the study was 101.17%、100.74%,RSD=1.84%、1.29%.Conclusion The results of simulation indicate that this method was accurate,reliable and reproducible.It was suggested that this method can be used to determine the content of total polysaccharides and triterpenoid saponin in the GLS.
