CAJ | 학술논문

目的：探讨人眼葡萄膜黑素瘤细胞系M23转染m i R-137后对细胞增殖和迁移能力的影响及其分子机制的初探。方法：采用阳离子脂质体LipofectamineTM2000将miR-137mimics转染M23细胞，应用CCK-8法检测转染后细胞增殖变化，划痕实验检测细胞迁移能力变化。Westernblot检测PTEN、总AKT和磷酸化AKT蛋白表达。结果：转染miR-137后，实验组的细胞增殖较对照组明显减低（t=-18.754，P=0.000；）；在36h和72h时间点，实验组细胞的划痕愈合程度均明显低于对照；实验组的PTEN蛋白表达增加，p-AKT蛋白减低，AKT蛋白表达无明显变化。结论：miR-137通过上调PTEN蛋白表达和减低AKT磷酸化活性从而抑制人眼葡萄膜黑素瘤细胞的增殖和迁移能力。
목적：탐토인안포도막흑소류세포계M23전염m i R-137후대세포증식화천이능력적영향급기분자궤제적초탐。방법：채용양리자지질체LipofectamineTM2000장miR-137mimics전염M23세포，응용CCK-8법검측전염후세포증식변화，화흔실험검측세포천이능력변화。Westernblot검측PTEN、총AKT화린산화AKT단백표체。결과：전염miR-137후，실험조적세포증식교대조조명현감저（t=-18.754，P=0.000；）；재36h화72h시간점，실험조세포적화흔유합정도균명현저우대조；실험조적PTEN단백표체증가，p-AKT단백감저，AKT단백표체무명현변화。결론：miR-137통과상조PTEN단백표체화감저AKT린산화활성종이억제인안포도막흑소류세포적증식화천이능력。
Objective To study the effects of miR-137 on proliferation and migration in human uveal malignant melanoma celllines M23 and investigate its action mechanisms.Methods miR-137 mimics were transfected M23 by LipofectamineTM 2000. The change of cellproliferation and migration of M23 cells were studied by cellCounting Kit-8 (CCK-8) assay and wound migration assay, respectively. PTEN, p-AKT and AKT proteins expression were investigated by western blot. Results miR-137 significantly inhibited cellproliferation and migration. PTEN protein was markedly increased and the phosphorylation of AKT was markedly reduced by miR-137 mimics treatment, respectively, but miR-137 was no effect on unphosphorylated AKT. Conclusion miR-137 exhibits inhibitory effects on proliferation and migration in human uveal melanoma cellthrough up-regulated PTEN protein expression and inhibit the phosphorylation of AKT.