中国医药导报
中國醫藥導報
중국의약도보
CHINA MEDICAL HERALD
2014年
20期
110-113
,共4页
谢谊%李跃辉%唐纯玉%唐代凤%刘玉琴%曹立云%彭艳梅
謝誼%李躍輝%唐純玉%唐代鳳%劉玉琴%曹立雲%彭豔梅
사의%리약휘%당순옥%당대봉%류옥금%조립운%팽염매
喉咽清口服液%齐墩果酸%酸水解%含量测定%高效液相色谱法
喉嚥清口服液%齊墩果痠%痠水解%含量測定%高效液相色譜法
후인청구복액%제돈과산%산수해%함량측정%고효액상색보법
Houyanqing Oral Liquids%Oleanolic acid%Acid hydrolysis%Content determination%HPLC
目的:建立HPLC-UV法测定酸水解后喉咽清口服液中的齐墩果酸的含量。方法以盐酸-乙醇溶液为水解溶剂,水浴加热回流1 h,再用石油醚(60~90℃)萃取得齐墩果酸;采用高效液相色谱法,以Ultimate XB-C18(4.6 mm×250 mm,5μm)色谱柱,乙腈-水(90:10)作为流动相,流速为1.0 mL/min,柱温为30℃,检测波长为210 nm。结果齐墩果酸线性范围为2~12μg,平均回收率99.09%,RSD为1.83%。结论所建立方法操作简便、准确、可靠、重现性好,可用于喉咽清口服液中齐墩果酸的含量测定。
目的:建立HPLC-UV法測定痠水解後喉嚥清口服液中的齊墩果痠的含量。方法以鹽痠-乙醇溶液為水解溶劑,水浴加熱迴流1 h,再用石油醚(60~90℃)萃取得齊墩果痠;採用高效液相色譜法,以Ultimate XB-C18(4.6 mm×250 mm,5μm)色譜柱,乙腈-水(90:10)作為流動相,流速為1.0 mL/min,柱溫為30℃,檢測波長為210 nm。結果齊墩果痠線性範圍為2~12μg,平均迴收率99.09%,RSD為1.83%。結論所建立方法操作簡便、準確、可靠、重現性好,可用于喉嚥清口服液中齊墩果痠的含量測定。
목적:건립HPLC-UV법측정산수해후후인청구복액중적제돈과산적함량。방법이염산-을순용액위수해용제,수욕가열회류1 h,재용석유미(60~90℃)췌취득제돈과산;채용고효액상색보법,이Ultimate XB-C18(4.6 mm×250 mm,5μm)색보주,을정-수(90:10)작위류동상,류속위1.0 mL/min,주온위30℃,검측파장위210 nm。결과제돈과산선성범위위2~12μg,평균회수솔99.09%,RSD위1.83%。결론소건립방법조작간편、준학、가고、중현성호,가용우후인청구복액중제돈과산적함량측정。
Objective To establish a method for the determination of oleanolic acid in Houyanqing Oral Liquids after being acid hydrolysis by HPLC. Methods The sample was hydrolyzed with hydrochloric acid- ethanol for 1 hours and extracted with petroleum ether (60-90℃);the HPLC method was adopted. The Ultimate XB-C18 column (4.6 mmí250 mm, 5μm) was used at the column temperature of 30℃. The mobile phase was composed of acetonitrile-water (90:10) at a flow rate of 1.0 mL/min, the detected wavelength was set at 210 nm. Results The linear ranges of oleanolic acid was 2-12 μg, the average recovery rate was 99.09% with the RSD was 1.83%. Conclusion The developed method is so con-venient and has better precision and repeatability. This method can be used to determine the content of oleanolic acid in Houyanqing Oral Liquids.