CAJ | 학술논문

目的：分析茁重型地中海贫血儿童NK细胞频率及杀伤活性与输血诱导的免疫抑制的相关性。方法收集2011年1月~2013年12月在广州市妇女儿童医疗中心及中山大学附属第三医院进行输血治疗长达3年的茁重型地中海贫血儿童20例(茁重型地中海贫血组)及健康儿童20例(对照组)，通过流式细胞技术检测其外周血NK细胞(CD3-CD56+)的频率及NK细胞杀伤活性(CD107a表达)。结果与对照组比较，长期输血的茁重型地中海贫血组儿童外周血NK细胞频率显著降低[(4.5±2.8)豫比(12.0±4.0)豫]，差异有统高度计学意义(P=0.000)。外周血CD107a表达(NK细胞的杀伤活性)显著下调[(23.9±7.4)豫比(51.4±11.9)豫]，差异有统高度计学意义(P=0.000)。结论茁重型地中海贫血组儿童外周血NK细胞频率的减少及NK细胞杀伤活性(CD107a表达)的减弱，可能与输血诱导的免疫抑制相关。
목적：분석촬중형지중해빈혈인동NK세포빈솔급살상활성여수혈유도적면역억제적상관성。방법수집2011년1월~2013년12월재엄주시부녀인동의료중심급중산대학부속제삼의원진행수혈치료장체3년적촬중형지중해빈혈인동20례(촬중형지중해빈혈조)급건강인동20례(대조조)，통과류식세포기술검측기외주혈NK세포(CD3-CD56+)적빈솔급NK세포살상활성(CD107a표체)。결과여대조조비교，장기수혈적촬중형지중해빈혈조인동외주혈NK세포빈솔현저강저[(4.5±2.8)예비(12.0±4.0)예]，차이유통고도계학의의(P=0.000)。외주혈CD107a표체(NK세포적살상활성)현저하조[(23.9±7.4)예비(51.4±11.9)예]，차이유통고도계학의의(P=0.000)。결론촬중형지중해빈혈조인동외주혈NK세포빈솔적감소급NK세포살상활성(CD107a표체)적감약，가능여수혈유도적면역억제상관。
Objective To analyze the association between the frequency and cytotoxicity of peripheral NK cells in β-thalassemia major children with the transfusion-induced immunodepression. Methods From January 2011 to December 2013, in Women and Children's Medical Center of Guangzhou City and the Third Affiliated Hospital of Sun Yat-sen University, the peripheral blood samples of 20 cases of β-thalassemia major children with 3 years blood transfusion (β-thalassemia major group) and 20 healthy children (control group) were obtained. The frequency of peripheral NK cells (CD3-CD56+) and CD107a expression on surface of peripheral NK cells were detected by flow cytometry. Results The proportion of peripheral NK cells of children with β-thalassemia major was markedly decreased than that of the control group [(4.5±2.8)% vs (12.0±4.0)%], the difference was statistically significant (P= 0.000). The expression of CD107a on the surface of CD3-CD56+NK cells from patients with β-thalassemia major was remarkably down regulated than that of the control group [(23.9±7.4)% vs (51.4±11.9)%],the difference was statistically significant (P=0.000). Conclusion The decreased frequency and the cytotoxicity (CD107a expression) of NK cells inβ-thalassemia major may contribute to the transfusion-induced immunodepression.