中国医药导报
中國醫藥導報
중국의약도보
CHINA MEDICAL HERALD
2014年
20期
16-19
,共4页
地西泮%血药浓度%一阶导数光谱法%治疗药物监测
地西泮%血藥濃度%一階導數光譜法%治療藥物鑑測
지서반%혈약농도%일계도수광보법%치료약물감측
Diazepam%Plasma drug concentration%First order derivative spectrophotometry%Therapeutic drug monitoring
目的：建立测定人血浆中地西泮浓度的一阶导数分光光度法，为临床应用提供技术参考。方法采用一阶导数光谱法在240.0 nm波长处测定人血浆中地西泮浓度。结果地西泮在5.35~31.85μg/mL范围呈良好线性关系；其回归方程为D=1.241×10-3C+3.0706×10-4(r=0.9979)；平均回收率为100.85%，RSD=1.88%(n=6)。结论该法操作简单，测定结果准确，适用于地西泮血药浓度的监测。
目的：建立測定人血漿中地西泮濃度的一階導數分光光度法，為臨床應用提供技術參攷。方法採用一階導數光譜法在240.0 nm波長處測定人血漿中地西泮濃度。結果地西泮在5.35~31.85μg/mL範圍呈良好線性關繫；其迴歸方程為D=1.241×10-3C+3.0706×10-4(r=0.9979)；平均迴收率為100.85%，RSD=1.88%(n=6)。結論該法操作簡單，測定結果準確，適用于地西泮血藥濃度的鑑測。
목적：건립측정인혈장중지서반농도적일계도수분광광도법，위림상응용제공기술삼고。방법채용일계도수광보법재240.0 nm파장처측정인혈장중지서반농도。결과지서반재5.35~31.85μg/mL범위정량호선성관계；기회귀방정위D=1.241×10-3C+3.0706×10-4(r=0.9979)；평균회수솔위100.85%，RSD=1.88%(n=6)。결론해법조작간단，측정결과준학，괄용우지서반혈약농도적감측。
Objective To develop first order derivative spectrophotometry for the determination of Diazepam concentra-tion in human plasma, to provide the technical reference for its clinical application. Methods The first order derivative spectrophotometry was performed at the wavelength of 240.0 nm. Results Diazepam showed a good linear relationship within a ranger of 5.35-31.85 μg/mL, the regression equation was D=1.241í10-3C+3.0706í10-4 (r=0.9979); the average recovery was 100.85% and RSD=1.88%(n=6). Conclusion This method is simple to operate and accurate in the detec-tion results, and can be applied in the analysis of Diazepam in human plasma.
