中国医药导报
中國醫藥導報
중국의약도보
CHINA MEDICAL HERALD
2014年
20期
9-12
,共4页
丹参总酚酸%山楂总黄酮%高脂血症%组分配伍
丹參總酚痠%山楂總黃酮%高脂血癥%組分配伍
단삼총분산%산사총황동%고지혈증%조분배오
Salvia miltiorrhizae total Salvianolic acid%Hawthorn flavonoids%Hyperlipidemia%Component compatibility
目的：探讨丹参总酚酸、山楂总黄酮组分配伍对高脂血症大鼠血脂、超氧化物歧化酶(SOD)及丙二醛(MDA)超氧化物歧化酶、丙二醛的影响。方法采用基线等比增减设计法重组丹参总酚酸、山楂总黄酮配伍，80只SD大鼠随机分为8组，每组10只，雌雄各半。正常组予以普通饲料喂养，丹参、山楂配伍A、B、C、D、E组和立普妥(降脂阳性对照组)组均给予高脂饲料(35 g/d)。丹参、山楂配伍A、B、C、D、E组分别剂量灌胃给予丹参总酚酸(mg/kg)：山楂总黄酮(mg/kg)=120：0、90：30、60：60、30：90、0：120，立普妥组按2.2 mg/kg灌胃给药，正常组和模型组均给予等体积生理盐水。连续给药至第30天，检测血清总胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)及MDA、SOD活性，并计算动脉粥样硬化指数(TC/HDL-C)。结果造模后模型组大鼠血清TC、TG、LDL-C水平、TC/HDL-C、MDA水平升高，HDL-C、SOD水平降低，与正常组相比差异均有高度统计学意义(均P<0.01)，模型复制成功。丹参酚酸和山楂黄酮按不同比例配伍后均可明显降低TC、TG、LDL-C、TC/HDL-C、MDA水平，提高HDL-C、SOD水平。其中C、D组降脂、抗氧化活性优于其他组，与模型组相比，差异有高度统计学意义(P<0.01)。结论丹参总酚酸、山楂总黄酮配伍可增强降脂和抗氧化效应，具有协同增效作用。
目的：探討丹參總酚痠、山楂總黃酮組分配伍對高脂血癥大鼠血脂、超氧化物歧化酶(SOD)及丙二醛(MDA)超氧化物歧化酶、丙二醛的影響。方法採用基線等比增減設計法重組丹參總酚痠、山楂總黃酮配伍，80隻SD大鼠隨機分為8組，每組10隻，雌雄各半。正常組予以普通飼料餵養，丹參、山楂配伍A、B、C、D、E組和立普妥(降脂暘性對照組)組均給予高脂飼料(35 g/d)。丹參、山楂配伍A、B、C、D、E組分彆劑量灌胃給予丹參總酚痠(mg/kg)：山楂總黃酮(mg/kg)=120：0、90：30、60：60、30：90、0：120，立普妥組按2.2 mg/kg灌胃給藥，正常組和模型組均給予等體積生理鹽水。連續給藥至第30天，檢測血清總膽固醇(TC)、三酰甘油(TG)、高密度脂蛋白膽固醇(HDL-C)、低密度脂蛋白膽固醇(LDL-C)及MDA、SOD活性，併計算動脈粥樣硬化指數(TC/HDL-C)。結果造模後模型組大鼠血清TC、TG、LDL-C水平、TC/HDL-C、MDA水平升高，HDL-C、SOD水平降低，與正常組相比差異均有高度統計學意義(均P<0.01)，模型複製成功。丹參酚痠和山楂黃酮按不同比例配伍後均可明顯降低TC、TG、LDL-C、TC/HDL-C、MDA水平，提高HDL-C、SOD水平。其中C、D組降脂、抗氧化活性優于其他組，與模型組相比，差異有高度統計學意義(P<0.01)。結論丹參總酚痠、山楂總黃酮配伍可增彊降脂和抗氧化效應，具有協同增效作用。
목적：탐토단삼총분산、산사총황동조분배오대고지혈증대서혈지、초양화물기화매(SOD)급병이철(MDA)초양화물기화매、병이철적영향。방법채용기선등비증감설계법중조단삼총분산、산사총황동배오，80지SD대서수궤분위8조，매조10지，자웅각반。정상조여이보통사료위양，단삼、산사배오A、B、C、D、E조화립보타(강지양성대조조)조균급여고지사료(35 g/d)。단삼、산사배오A、B、C、D、E조분별제량관위급여단삼총분산(mg/kg)：산사총황동(mg/kg)=120：0、90：30、60：60、30：90、0：120，립보타조안2.2 mg/kg관위급약，정상조화모형조균급여등체적생리염수。련속급약지제30천，검측혈청총담고순(TC)、삼선감유(TG)、고밀도지단백담고순(HDL-C)、저밀도지단백담고순(LDL-C)급MDA、SOD활성，병계산동맥죽양경화지수(TC/HDL-C)。결과조모후모형조대서혈청TC、TG、LDL-C수평、TC/HDL-C、MDA수평승고，HDL-C、SOD수평강저，여정상조상비차이균유고도통계학의의(균P<0.01)，모형복제성공。단삼분산화산사황동안불동비례배오후균가명현강저TC、TG、LDL-C、TC/HDL-C、MDA수평，제고HDL-C、SOD수평。기중C、D조강지、항양화활성우우기타조，여모형조상비，차이유고도통계학의의(P<0.01)。결론단삼총분산、산사총황동배오가증강강지화항양화효응，구유협동증효작용。
Objective To observe the effect on hyperlipidemia rats of blood lipid, SOD, MDA with Radix Salvia miltior-rhizae total Salvianolic acid and hawthorn flavonoids. Methods The ratio between Salvia miltiorrhizae total Salvianolic acid and hawthorn flavonoids was recombined with increase-decrease baseline geometric proportion design method. 80 SD rats were randomly divided into eight groups, each group of 10 with only male and female half and half. Normal group was given ordinary feed, salvia miltiorrhiza, hawthorn compatibility A, B, C, D, E group and lipitor (lipid-lower-ing positive control group) were given high-fat feed (35 g/d). Salvia miltiorrhiza, hawthorn compatibility A, B, C, D, E group were given Salvia miltiorrhizae total Salvianolic acid (mg/kg):hawthorn flavonoids (mg/kg)=120:0, 30:90, 30:60, 60:90, 0:120, the lipitor group was 2.2 mg/kg to fill the stomach, the normal group and model group were given normal saline volume. Dosing to 30 days in a row, serum total cholesterol (TC), three acyl glycerin (TG), high-density lipopro-tein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C) and MDA, SOD activity were detected, and the index of atherosclerosis (TC/HDL-C) was calculated. Results Compared with normal group, the blood lipid parameters and SOD, MDA level of model group were significantly different (P< 0.01), TC, TG, LDL-C, TC/HDL-C and MDAmarkably increase the concentration of serum HDL-C and the activity of SOD in the hyperlipemia rats. Compared with hyperlipemia model group, the lipid-lowering and antioxidant activity in C and D group was better (P<0.01). Conclu-sion The Salvia miltiorrhizae total Salvianolic acid and hawthorn flavonoids have the potent hypolipidemic and antioxi-dant action which show synergistic effect.
