CAJ | 학술논문

探讨eIF4E对厄洛替尼耐药细胞株细胞增殖的影响及作用机制.在配对的厄洛替尼敏感和耐药细胞株( HCC827-EP、HCC827-ER)上,采用实时定量PCR检测mRNA水平的变化,Western Blot检测蛋白水平的变化；通过SRB检测细胞的生长和药物的抑制率的变化.以及用脂质体转染eIF4E的小干扰RNA,用上述方法检测多种信号分子mRNA、蛋白水平的变化,及对细胞生长和对药物抑制率的影响.结果显示,与HCC827-EP相比,eIF4E在HCC827-ER上高表达；HCC827-ER转染eIF4E siRNA的细胞生长速度明显减慢,从第3 d起差异具有统计学意义(P<0.05),在相同浓度的厄洛替尼作用下,转染eIF4E siRNA的细胞具有更高的抑制率,差异具有统计学意义(P<0.05).结论：下调eIF4E的表达能够增强厄洛替尼对NSCLC的抑制作用,eIF4E的高表达参与了厄洛替尼获得性耐药的产生.
탐토eIF4E대액락체니내약세포주세포증식적영향급작용궤제.재배대적액락체니민감화내약세포주( HCC827-EP、HCC827-ER)상,채용실시정량PCR검측mRNA수평적변화,Western Blot검측단백수평적변화；통과SRB검측세포적생장화약물적억제솔적변화.이급용지질체전염eIF4E적소간우RNA,용상술방법검측다충신호분자mRNA、단백수평적변화,급대세포생장화대약물억제솔적영향.결과현시,여HCC827-EP상비,eIF4E재HCC827-ER상고표체；HCC827-ER전염eIF4E siRNA적세포생장속도명현감만,종제3 d기차이구유통계학의의(P<0.05),재상동농도적액락체니작용하,전염eIF4E siRNA적세포구유경고적억제솔,차이구유통계학의의(P<0.05).결론：하조eIF4E적표체능구증강액락체니대NSCLC적억제작용,eIF4E적고표체삼여료액락체니획득성내약적산생.
To observe the effects of eIF4E on the proliferation of erlotinib resistant cells and mechanism. Paired erlotinib sensitive and resistant HCC827-EP and HCC827-ER cells were used. Protein and mRNA expression levels were detected by Western blot and quantitative RT-PCR. The 5 ds growth curve and inhibition effects of erlotinib were determined by SRB assay. Downregulation of eIF4E expression were achieved by transfection of cells with siRNA targeting eIF4E using lipofectamine,and nonsense siRNA were used as control. eIF4E expression was increased in HCC827-ER cells compared with HCC827-EP cells. And downregulation of eIF4E expression inhibited the growth of HCC827-ER cells(P<0. 05)and sensitized their reponses erlotinib possibly through downregualtion of eIF4E expression(P<0. 05). Downregulation of eIF4E expression inhibited the growth of NSCLCs and enhanced the inhibitory effects of erlotinib.