CAJ | 학술논문

从毛冠鹿睾丸cDNA文库中筛选出毛冠鹿AIF-1基因,对其进行生物信息学分析,设计引物克隆毛冠鹿AIF-1 cDNA,连入pMD19-T载体,测序正确后酶切,与表达载体pET-28a(+)连接,转化E. coli BL21(DE3), IPTG诱导表达,将诱导表达重组蛋白的菌体超声破碎后,进行可溶性分析,并对可溶性蛋白进行纯化, SDS-PAGE电泳,Western Blot分析以及鉴定重组蛋白.结果表明,毛冠鹿AIF-1(TdAIF-1)含有1个438bp的开放阅读框,编码145个氨基酸,经测序和酶切鉴定后,成功构建重组质粒pET-28a(+)-TdAIF-1,表达大小约20 kD的重组蛋白,主要以可溶形式存在,提高洗脱缓冲液中咪唑浓度至50 mmol/L、100 mmol/L能够得到较纯的蛋白.成功构建毛冠鹿AIF-1原核表达体系,获得重组蛋白,为研究AIF-1蛋白的生物学功能奠定了基础.
종모관록고환cDNA문고중사선출모관록AIF-1기인,대기진행생물신식학분석,설계인물극륭모관록AIF-1 cDNA,련입pMD19-T재체,측서정학후매절,여표체재체pET-28a(+)련접,전화E. coli BL21(DE3), IPTG유도표체,장유도표체중조단백적균체초성파쇄후,진행가용성분석,병대가용성단백진행순화, SDS-PAGE전영,Western Blot분석이급감정중조단백.결과표명,모관록AIF-1(TdAIF-1)함유1개438bp적개방열독광,편마145개안기산,경측서화매절감정후,성공구건중조질립pET-28a(+)-TdAIF-1,표체대소약20 kD적중조단백,주요이가용형식존재,제고세탈완충액중미서농도지50 mmol/L、100 mmol/L능구득도교순적단백.성공구건모관록AIF-1원핵표체체계,획득중조단백,위연구AIF-1단백적생물학공능전정료기출.
The TdAIF-1 cDNA was cloned from the testis cDNA library of the Tufted deer( Elaphodus cephalophus) and analyzed by bioinformatic methods. Primers were designed according to cDNA sequence to clone the gene. The gene was cloned into pMD19-T vector for sequencing. The right sequence was digested by restriction enzyme and subcloned into the expression vector pET-28a(+). After transformed into E. coli BL21(DE3),the recombinant plasimid was induced to express by IPTG. The E. coli BL21(DE3)expressed recombinant protein was broken by ultrasonic to show whether the re-combinant protein was soluble or not. Lastly,the soluble protein was purified. The recombinant protein was analyzed and identificated by SDS-PAGE electrophoresis and Western blot. Analysis of sequence showed that the TdAIF-1 cDNA contained a 438 bp open reading frame encoding 145 amino acids. The recombinant plasimid was correctly constructed according to sequencing and restriction enzyme analysis. The recombinant protein was about 20 kD and soluble mainly. When the recombinant protein was purified,using elution buffer containing 50 mmol/L or 100 mmol/L imidazole could get purified protein. The TdAIF-1 Prokaryotic Expression System was constructed successfully and recombinant protein was obtained,which was helpful for the future study of its biological function.