中医药信息
中醫藥信息
중의약신식
INFORMATION ON TRADITIONAL CHINESE MEDICINE
2014年
2期
60-61,62
,共3页
冯宇飞%井中旭%王艳宏%吕邵娃%李永吉
馮宇飛%井中旭%王豔宏%呂邵娃%李永吉
풍우비%정중욱%왕염굉%려소왜%리영길
MTT法%双黄连粉针%组成药材提取物%主要有效成分%IC50
MTT法%雙黃連粉針%組成藥材提取物%主要有效成分%IC50
MTT법%쌍황련분침%조성약재제취물%주요유효성분%IC50
MTT method%Shuanghuanglian powder%Herbal extracts%Main effective components%IC50
目的：采用MTT法测定双黄连粉针中三种组成药材提取物及三种主要有效成分对大鼠腹腔肥大细胞IC50。方法：将空白大鼠腹腔肥大细胞无菌条件下分离纯化后，高、中、低三个浓度与腹腔肥大细胞及一定量的MTT共同孵育后，测定490 nm处OD值。结果：双黄连中三种药材提取物及主要有效成分对大鼠肥大细胞的IC50分别为：0．18mg／mL、12．0μg／mL、0．06mg／mL；0．5mg／mL、8.0μg／mL、0.04mg／mL。结论：通过对上述各组药物对大鼠腹腔肥大细胞的IC50的测定，为考察双黄连粉针中各物质对肥大细胞的脱颗粒作用奠定实验基础。
目的：採用MTT法測定雙黃連粉針中三種組成藥材提取物及三種主要有效成分對大鼠腹腔肥大細胞IC50。方法：將空白大鼠腹腔肥大細胞無菌條件下分離純化後，高、中、低三箇濃度與腹腔肥大細胞及一定量的MTT共同孵育後，測定490 nm處OD值。結果：雙黃連中三種藥材提取物及主要有效成分對大鼠肥大細胞的IC50分彆為：0．18mg／mL、12．0μg／mL、0．06mg／mL；0．5mg／mL、8.0μg／mL、0.04mg／mL。結論：通過對上述各組藥物對大鼠腹腔肥大細胞的IC50的測定，為攷察雙黃連粉針中各物質對肥大細胞的脫顆粒作用奠定實驗基礎。
목적：채용MTT법측정쌍황련분침중삼충조성약재제취물급삼충주요유효성분대대서복강비대세포IC50。방법：장공백대서복강비대세포무균조건하분리순화후，고、중、저삼개농도여복강비대세포급일정량적MTT공동부육후，측정490 nm처OD치。결과：쌍황련중삼충약재제취물급주요유효성분대대서비대세포적IC50분별위：0．18mg／mL、12．0μg／mL、0．06mg／mL；0．5mg／mL、8.0μg／mL、0.04mg／mL。결론：통과대상술각조약물대대서복강비대세포적IC50적측정，위고찰쌍황련분침중각물질대비대세포적탈과립작용전정실험기출。
Objective:IC50 was determined by MTT method of three composed species of herbal extracts and three kinds of main effective components in Shuanghuanglian powder affecting rat peritoneal mast cells .Meth-ods:Separate the blank rat peritoneal mast cells under aseptic condition after purification , high, middle and low concentrations of each subject and peritoneal mast cells with a certain amount of MTT were incubated ,and the OD value was determined in 490 nm.Results: IC50 of three composed species of herbal extracts and three kinds of main effective components in Shuanghuanglian powder was 0.18mg/mL,12.0μg/mL,0.06mg/mL;0. 5mg/mL,8.0μg/mL,0.04mg/mL respectively .Conclusion:Through the determination of the IC 50 of different groups on rat peritoneal mast cells , it is in order to provide experimental basis for testing each substance in Shuanghuanglian powder on mast cell degranulation .
