CAJ | 학술논문

目的：观察清火解毒法内服方和外用熏蒸方对乙型溶血性链球菌的体内外抑菌作用。方法：体外抑菌实验采用增菌培养和二倍稀释法，体内抑菌实验采用清洁级ICR小鼠腹腔注射乙型链球菌培养原液0．5 ml／只，连续给药2天，观察死亡率和存活天数。结果：受试药对标准菌株和临床分离菌均具有较强的抑菌作用，MIC为16．5～156 mg／ml；清火解毒法内服方高、中剂量组对乙型链球菌感染小鼠均可降低其死亡率，延长存活天数，与模型组比较差异有统计学意义（P＜0．01，P＜0．05）。结论：清火解毒法对乙型溶血性链球菌体内外均有抗菌作用。
목적：관찰청화해독법내복방화외용훈증방대을형용혈성련구균적체내외억균작용。방법：체외억균실험채용증균배양화이배희석법，체내억균실험채용청길급ICR소서복강주사을형련구균배양원액0．5 ml／지，련속급약2천，관찰사망솔화존활천수。결과：수시약대표준균주화림상분리균균구유교강적억균작용，MIC위16．5～156 mg／ml；청화해독법내복방고、중제량조대을형련구균감염소서균가강저기사망솔，연장존활천수，여모형조비교차이유통계학의의（P＜0．01，P＜0．05）。결론：청화해독법대을형용혈성련구균체내외균유항균작용。
Objective:To observe the inhibitory effect of in vitro and vivo on Beta -hemolytic streptococcus by Qinghuo decoction oral side and topical fumigation party .Methods: The inhibitive experiments in vitro used enrichment culture and the double dilution method .The inhibitive experiments in vivo used ICR mice of clean grade injected beta-hemolytic streptococcus culture liquid 0.5ml/only, then continued administration for two days .Results:The investigational drug had a strongly inhibitory effect on the standard strains and clinical iso-lates, MIC at 16.5mg~156mg/ml.The high dose group of Qinghuo decoction oral side could reduce the mor-tality rate for beta-hemolytic streptococcal infection in mice , and could prolong survival days .It was com-pared with the model group ,and the difference was statistically significant (P<0.01,P<0.05).Conclusion:The clear fire detoxification party has an antibacterial effect in vitro and in vivo on beta -hemolytic streptococ-cus .