CAJ | 학술논문

目的：比较橙皮素和橙皮苷与牛血清白蛋白（ BSA ）作用的异同，探讨黄酮类化合物A环7位羟基糖苷化与否对结合血清白蛋白的影响。方法：荧光光谱、紫外-可见吸收光谱法测定橙皮素和橙皮苷与BSA的作用。结果：橙皮素对BSA的荧光猝灭效率明显高于橙皮苷；橙皮素对BSA的荧光猝灭以静态猝灭为主，而橙皮苷对BSA的荧光猝灭既有静态猝灭作用，也有动态猝灭作用；橙皮素与BSA结合的Ka值明显大于橙皮苷；橙皮素结合BSA的位点与荧光发射基团的距离比橙皮苷的小；橙皮素与BSA之间的作用主要是氢键和范德华力，而橙皮苷与BSA的作用主要是疏水作用力。结论：橙皮素与BSA的结合能力明显强于橙皮苷，说明橙皮素比橙皮苷更容易被血清蛋白贮存和运输。
목적：비교등피소화등피감여우혈청백단백（ BSA ）작용적이동，탐토황동류화합물A배7위간기당감화여부대결합혈청백단백적영향。방법：형광광보、자외-가견흡수광보법측정등피소화등피감여BSA적작용。결과：등피소대BSA적형광졸멸효솔명현고우등피감；등피소대BSA적형광졸멸이정태졸멸위주，이등피감대BSA적형광졸멸기유정태졸멸작용，야유동태졸멸작용；등피소여BSA결합적Ka치명현대우등피감；등피소결합BSA적위점여형광발사기단적거리비등피감적소；등피소여BSA지간적작용주요시경건화범덕화력，이등피감여BSA적작용주요시소수작용력。결론：등피소여BSA적결합능력명현강우등피감，설명등피소비등피감경용역피혈청단백저존화운수。
Objective:To compare the similarities and differences in interaction of hesperetin and hesperidin with bovine serum albumin ( BSA ) .Methods: Fluorescence spectra , UV Vis absorption spectroscopy method were applied to investigate the interaction of Luteolin and Quercetin with BSA .Results: Quenching fluores-cence efficiency of BSA by hesperetin was stronger than that of hesperidin .The fluorescence quenching of hes-peretin on BSA was mainly a static quenching ,but the fluorescence quenching of hesperidin on BSA was both static and dynamic quenching .The binding constants ( Ka ) of hesperetin with BSA were significantly greater than that of hesperidin .The binding distance between hesperetin and BSA was shorter than that of hesperidin . The binding force of hesperetin to BSA was mainly the hydrogen bonds and van der Waals forces , and that of hesperidin to BSA was mainly hydrophobic interaction .Conclusion:The binding ability to BSA of hesperetin is stronger than that of hesperidin .It shows that the hesperetin is more easily storaged and transported by serum protein than that of hesperidin .