CAJ | 학술논문

基于微生物诱导碳酸钙沉积的岩土工程加固技术是一种环境友好的新技术。碳酸钙矿化菌是该技术应用的前提。为获得具有诱导碳酸钙沉积能力的菌株，采用选择性富集培养、平板分离方法从土壤中分离得到了一株具有尿素分解能力的菌株，细菌诱导产生的沉积物检测结果表明该菌株具有诱导碳酸钙沉积能力。通过形态学、革兰氏染色和16 S rDNA序列同源性分析鉴定该菌株为巴斯德芽孢杆菌。
기우미생물유도탄산개침적적암토공정가고기술시일충배경우호적신기술。탄산개광화균시해기술응용적전제。위획득구유유도탄산개침적능력적균주，채용선택성부집배양、평판분리방법종토양중분리득도료일주구유뇨소분해능력적균주，세균유도산생적침적물검측결과표명해균주구유유도탄산개침적능력。통과형태학、혁란씨염색화16 S rDNA서렬동원성분석감정해균주위파사덕아포간균。
Biocementation through microbial calcium carbonate precipitation is an innova-tive and environmentally friendly rock and soil reinforcement technique in geotechnical en-gineering. The bacteria inducing mineralization of calcium carbonate is a prerequisite to im-plement the biological treatment process. In order to obtain the strain with ability to induce CaCO3 precipitation,a ureolytic strain was isolated from soil using selective enrichment cul-ture and plate screening techniques. The precipites induced by this stain were examined, and the results showed it was capable of inducing calcium carbonate mineralization. The strain was identified as Sporosarcina pasteurii based on morphology,Gram stain and 16S rDNA sequence analysis.