CAJ | 학술논문

目的：探讨三七理血脂肝方对大鼠非酒精性单纯性脂肪肝（ non-alcoholic fatty liver ， NAFL）的治疗作用，进而探讨其作用机制。方法：雄性 Wistar 大鼠36只，随机分成正常组（ C）、模型组（ M）、三七理血脂肝方组（ Y），每组12只。 C组给予普通饲料， M和Y组予高脂饲料造模，4周后继续予高脂饲料同时Y组大鼠予相应药物干预4周。动物实验结束时，取3组大鼠肝脏组织用苏木精-伊红染色、油红O染色进行病理学观察，检测肝组织甘油三酯（TG）、总胆固醇（TC）含量。检测血清丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）的活性，以及血脂血糖指标TG、 TC、低密度脂蛋白胆固醇（ LDL-C）、高密度脂蛋白胆固醇（ HDL-C）、游离脂肪酸（ FFA）、空腹血糖（FBG）。蛋白免疫印迹法检测肝组织细胞色素P450亚型2E1（cytochrome P4502E1， CYP2E1）和解耦联蛋白2（uncoupling protein-2， UCP2）的表达水平。结果： M组大鼠血清ALT和LDL-C 较C组显著升高， Y组中这两个指标较M组明显下调，并能降低血清FFA水平； M组大鼠肝组织出现弥漫性大泡性脂肪变性，肝组织 TG、 TC含量显著升高， Y组大鼠肝脏的脂肪变性从分布和程度上均有所减轻，肝脂含量降低。另外M组大鼠肝脏的CYP2E1的表达上调、 UCP2下调， Y组表达趋向正常水平。结论：三七理血脂肝方对大鼠NAFL治疗有效，方药的降脂理血功效可能通过抑制氧化应激、调节脂质代谢发挥降脂保肝作用。
목적：탐토삼칠리혈지간방대대서비주정성단순성지방간（ non-alcoholic fatty liver ， NAFL）적치료작용，진이탐토기작용궤제。방법：웅성 Wistar 대서36지，수궤분성정상조（ C）、모형조（ M）、삼칠리혈지간방조（ Y），매조12지。 C조급여보통사료， M화Y조여고지사료조모，4주후계속여고지사료동시Y조대서여상응약물간예4주。동물실험결속시，취3조대서간장조직용소목정-이홍염색、유홍O염색진행병이학관찰，검측간조직감유삼지（TG）、총담고순（TC）함량。검측혈청병안산안기전이매（ALT）、천동안산안기전이매（AST）적활성，이급혈지혈당지표TG、 TC、저밀도지단백담고순（ LDL-C）、고밀도지단백담고순（ HDL-C）、유리지방산（ FFA）、공복혈당（FBG）。단백면역인적법검측간조직세포색소P450아형2E1（cytochrome P4502E1， CYP2E1）화해우련단백2（uncoupling protein-2， UCP2）적표체수평。결과： M조대서혈청ALT화LDL-C 교C조현저승고， Y조중저량개지표교M조명현하조，병능강저혈청FFA수평； M조대서간조직출현미만성대포성지방변성，간조직 TG、 TC함량현저승고， Y조대서간장적지방변성종분포화정도상균유소감경，간지함량강저。령외M조대서간장적CYP2E1적표체상조、 UCP2하조， Y조표체추향정상수평。결론：삼칠리혈지간방대대서NAFL치료유효，방약적강지리혈공효가능통과억제양화응격、조절지질대사발휘강지보간작용。
Objective: To investigate the therapeutic efficacy of Sanqi Lixuezhigan recipe in the rat model of non -alcoholic fatty liver ( NAFL) , as well as the underlying mechanism.Methods: Thirty-six male Wistar rats were randomly divided into normal control group ( C) , model group ( M) and Sanqi Lixuezhigan recipe group ( Y) .The rats of control group was fed with normal diet, M group and Y group with high-fat diet for 8 weeks, and from the fifth week rats of Y group were also administrated with Sanqi Lixuezhigan recipe once a day.Serum alanine aminotransferase ( ALT) , aspartate aminotransferase ( AST) , triglycer-ides ( TG) , total cholesterol ( TC) , LDL-C, HDL-C, FFA and FBG were measured.Liver histopathology was observed through hematoxylin-eosin staining (HE stain) and oil red O staining.Liver TG and TC were also determined.In addition, the protein expression of liver CYP2E1 and UCP2 was detected by Western blot.Results: Serum ALT and LDL-C in the model rat increased obviously , while impaired again in the rat of Sanqi Lixuezhigan recipe treatment group.In the liver of M group , diffusing hepatic steatosis was observed in liver lobules , and the liver TG and TC level increased dramatically.Compared with model , less area and extent of steatosis were found in the rat liver of Y group.And serum FFA content also decreased in Y group.Western blot showed up-regulated expression of CYP2E1 and down-regulated expression of UCP2 in the liver of M group, while their expression tended to the normal levels in the liver tissue of treatment group.Conclusion: Sanqi Lixuezhigan recipe can effectively improve non-alcoholic fatty liver in the high fat diet induced rat model.The lipid-lowering and blood-regulating function for this complex recipe may enable it to ameliorate liver lipid accumulation and liver injury through inhibiting oxidative stress and improve lipid metabolism.