CAJ | 학술논문

目的：研究氧化苦参碱对慢性乙型肝炎病毒（ HBV ）携带者外周血树突状细胞及细胞因子的影响。方法：分离60例慢性HBV携带者外周血单核细胞后在诱导培养树突状细胞过程中治疗组加入氧化苦参碱，对照组不加，每组各30例，然后提取两组成熟的树突状细胞，计数后采用双标记流式细胞分析技术检测其活性，再用表位多肽刺激后，和自体淋巴细胞共育，分别检测IL-2和IFN-γ（ Th1分泌）及IL-4和IL-10（ Th2分泌）。结果：治疗组其树突状细胞数目增多，共刺激分子CD1a、 CD80、 CD86明显高于对照组（P＜0.05）。与自体淋巴细胞共育后产生的IL-2、 IFN-γ明显高于对照组（P＜0.05）； IL-4、 IL-10与对照组差异无显著性意义（P＞0.05）。结论：氧化苦参碱可增多、增强HBV携带者外周血树突状细胞的活性，当其与自体淋巴细胞共育时，可增强Th1类细胞因子的表达，对Th2类细胞因子无明显影响。
목적：연구양화고삼감대만성을형간염병독（ HBV ）휴대자외주혈수돌상세포급세포인자적영향。방법：분리60례만성HBV휴대자외주혈단핵세포후재유도배양수돌상세포과정중치료조가입양화고삼감，대조조불가，매조각30례，연후제취량조성숙적수돌상세포，계수후채용쌍표기류식세포분석기술검측기활성，재용표위다태자격후，화자체림파세포공육，분별검측IL-2화IFN-γ（ Th1분비）급IL-4화IL-10（ Th2분비）。결과：치료조기수돌상세포수목증다，공자격분자CD1a、 CD80、 CD86명현고우대조조（P＜0.05）。여자체림파세포공육후산생적IL-2、 IFN-γ명현고우대조조（P＜0.05）； IL-4、 IL-10여대조조차이무현저성의의（P＞0.05）。결론：양화고삼감가증다、증강HBV휴대자외주혈수돌상세포적활성，당기여자체림파세포공육시，가증강Th1류세포인자적표체，대Th2류세포인자무명현영향。
Objective: To research the effects of oxymatrine on the peripheral blood dendritic cells and cytokines in the pa -tients with chronic hepatitis B.Methods: Sixty patients with chronic hepatitis B were randomly divided into adding oxymatrine group (the treatment group, 30 cases) and without oxymatrine group (the control group , 30 cases), whose peripheral blood monouclear cells were isolated before the induction and culture of dendritic cells , oxymatrine added in the treatment group and no in the control group.The mature dendritic cells were extracted in the two groups , the activity of which were detected by the flow cytometry analysis using double labeling technique.After being stimulated by the epitope peptide , the dendritic cells and the au-tologous lymphocytes were cocultured , by which concentration of IL-2, IFN-γand IL-4, IL-10 were determined.Results: The number and activity of dendritic cells in the treatment group (adding oxymatrine group) increased (P<0.05) .After the den-dritic cells and the autologous lymphocytes were cocultured.The generated concentration of IL-2 IFN-γwere higher compared with those of the control group (P<0.05) .The generated concentration of IL-4 IL-10 were no change compared with those of the control group ( P>0.05) .Conclusion: Oxymatrine may enhance the number and activity of dendritic cells in the peripheral blood of the patients with chronic hepatitis B .The expression of Th1 type cytokines may be increased by the coculture of both the dendritic cells and the autologous lymphocytes.The expression of Th2 type cytokines may be no change by the coculture of both the dendritic cells and the autologous lymphocytes.