中国医师进修杂志
中國醫師進脩雜誌
중국의사진수잡지
CHINESE JOURNAL OF POSTGRADUATES OF MEDICINE
2014年
27期
7-9
,共3页
甲胎蛋白类%糖尿病,妊娠%胎盘%胰岛素抵抗
甲胎蛋白類%糖尿病,妊娠%胎盤%胰島素牴抗
갑태단백류%당뇨병,임신%태반%이도소저항
Alpha-fetoproteins%Diabetes,gestational%Placenta%Insulin resistance
目的 探讨妊娠期糖尿病(GDM)患者胎盘组织中胎球蛋白A(AHSG)基因mRNA及蛋白表达与胰岛素抵抗的关系.方法 采用反转录聚合酶链反应(RT-PCR)、Western blot技术检测43例正常足月妊娠剖宫产患者(对照组)和45例GDM患者(GDM组)胎盘组织中AHSG基因mRNA及蛋白表达水平,并检测剖宫产前空腹血糖(FPG)、空腹胰岛素(FINS),计算稳态模型评估法胰岛素抵抗指数(HOMA-IR).结果 GDM组FPG、FINS和HOMA-IR水平均显著高于对照组[(5.28±0.63) mmol/L比(4.24±0.21) mmol/L,(11.75±4.85) mU/L比(9.73±2.18) mU/L,2.79±1.02比1.87±0.66],差异有统计学意义(P＜0.01或＜0.05);GDM组胎盘组织AHSG基因mRNA和蛋白表达水平显著高于对照组(0.7616±0.0691比0.3294±0.025 1,0.824 5±0.1375比0.420 3±0.075 0),差异有统计学意义(P＜0.01或＜0.05);GDM组HOMA-IR水平与胎盘组织AHSG基因mRNA和蛋白表达水平呈正相关(r=0.633,0.587,P＜ 0.05).结论 GDM患者胎盘组织中AHSG基因mRNA和蛋白表达水平显著升高,胰岛素抵抗在GDM患者发病中有重要作用.
目的 探討妊娠期糖尿病(GDM)患者胎盤組織中胎毬蛋白A(AHSG)基因mRNA及蛋白錶達與胰島素牴抗的關繫.方法 採用反轉錄聚閤酶鏈反應(RT-PCR)、Western blot技術檢測43例正常足月妊娠剖宮產患者(對照組)和45例GDM患者(GDM組)胎盤組織中AHSG基因mRNA及蛋白錶達水平,併檢測剖宮產前空腹血糖(FPG)、空腹胰島素(FINS),計算穩態模型評估法胰島素牴抗指數(HOMA-IR).結果 GDM組FPG、FINS和HOMA-IR水平均顯著高于對照組[(5.28±0.63) mmol/L比(4.24±0.21) mmol/L,(11.75±4.85) mU/L比(9.73±2.18) mU/L,2.79±1.02比1.87±0.66],差異有統計學意義(P＜0.01或＜0.05);GDM組胎盤組織AHSG基因mRNA和蛋白錶達水平顯著高于對照組(0.7616±0.0691比0.3294±0.025 1,0.824 5±0.1375比0.420 3±0.075 0),差異有統計學意義(P＜0.01或＜0.05);GDM組HOMA-IR水平與胎盤組織AHSG基因mRNA和蛋白錶達水平呈正相關(r=0.633,0.587,P＜ 0.05).結論 GDM患者胎盤組織中AHSG基因mRNA和蛋白錶達水平顯著升高,胰島素牴抗在GDM患者髮病中有重要作用.
목적 탐토임신기당뇨병(GDM)환자태반조직중태구단백A(AHSG)기인mRNA급단백표체여이도소저항적관계.방법 채용반전록취합매련반응(RT-PCR)、Western blot기술검측43례정상족월임신부궁산환자(대조조)화45례GDM환자(GDM조)태반조직중AHSG기인mRNA급단백표체수평,병검측부궁산전공복혈당(FPG)、공복이도소(FINS),계산은태모형평고법이도소저항지수(HOMA-IR).결과 GDM조FPG、FINS화HOMA-IR수평균현저고우대조조[(5.28±0.63) mmol/L비(4.24±0.21) mmol/L,(11.75±4.85) mU/L비(9.73±2.18) mU/L,2.79±1.02비1.87±0.66],차이유통계학의의(P＜0.01혹＜0.05);GDM조태반조직AHSG기인mRNA화단백표체수평현저고우대조조(0.7616±0.0691비0.3294±0.025 1,0.824 5±0.1375비0.420 3±0.075 0),차이유통계학의의(P＜0.01혹＜0.05);GDM조HOMA-IR수평여태반조직AHSG기인mRNA화단백표체수평정정상관(r=0.633,0.587,P＜ 0.05).결론 GDM환자태반조직중AHSG기인mRNA화단백표체수평현저승고,이도소저항재GDM환자발병중유중요작용.
Objective To investigate the relationship between expressions of alpha 2-HS-glycoprotein (AHSG) gene mRNA and protein in placenta and insulin resistance in patients with gestational diabetes mellitus (GDM).Methods The expressions of AHSG gene mRNA and protein in placenta were detected by reverse transcriptase-polymerase chain reaction (RT-PC R) and Western blot technique in normal uterogestation uterine-incision delivery patients (control group,43 cases) and patients with GDM (GDM group,45 cases).Fasting plasma glucose (FPG) and fasting insuhn (FINS) were detected before uterine-incision delivery,and the homeostasis model of assessment for insulin resistance index (HOMA-IR) was computed.Results The FPG,FINS and HOMA-IR in GDM group were significantly higher than those in control group [(5.28 ± 0.63)mmol/L vs.(4.24 ±0.21) mmol/L,(11.75 ±4.85) mU/L vs.(9.73 ±2.18) mU/L,2.79 ±1.02 vs.1.87 ± 0.66],there were statistical differences (P ＜ 0.01 or ＜ 0.05).The expressions of AHSG gene mRNA and protein in GDM group were significantly higher than those in control group (0.761 6 ± 0.069 1 vs.0.329 4 ± 0.025 1 and 0.824 5 ± 0.137 5 vs.0.420 3 ± 0.075 0),there were statistical differences (P＜ 0.01or ＜ 0.05).HOMA-IR level in GDM group was positively correlated with AHSG gene mRNA and protein (r =0.633,0.587,P ＜0.05).Conclusions The expressions of AHSG gene mRNA and protein are significantly higher in patients with GDM.Insulin resistance may play an important role in the pathogenesis and development of GDM.
