CAJ | 학술논문

LSD1基因家族是一类特殊的 C2C2型锌指蛋白基因.OsLSD2是LSD1型基因家族的一员.已知 LSD1基因参与了植物抗病信号途径.为了培育环境友好型水稻,我们研究了 OsLSD2在日本晴中的过量表达.T3代转基因水稻表型表现为株高降低,分蘖数平均减少2个,结实率只有64％~72％,单株产量下降33％~42％,生育期提前15 d,对氮素的吸收利用率降低等,说明该基因过量表达显著抑制水稻的生长和氮素转运.
LSD1기인가족시일류특수적 C2C2형자지단백기인.OsLSD2시LSD1형기인가족적일원.이지 LSD1기인삼여료식물항병신호도경.위료배육배경우호형수도,아문연구료 OsLSD2재일본청중적과량표체.T3대전기인수도표형표현위주고강저,분얼수평균감소2개,결실솔지유64％~72％,단주산량하강33％~42％,생육기제전15 d,대담소적흡수이용솔강저등,설명해기인과량표체현저억제수도적생장화담소전운.
OsLSD2 is a member of LSD1 gene family,which encodes C2C2 zinc finger protein gene.As it is known that LSD1 family is involved in disease resistance.In order to create environment-friendly rice plant,we investigated the effects of overexpression of this gene in Nipponbare (WT)on plant growth and nitrogen use efficiency.The T3 generation plants showed that the plant height was reduced significantly;tiller number was averagely decreased by two;seed setting rate was only 64% - 72%;the grain yield was reduced by 33% -42% compared with WT;the growth duration was shortened by 1 5 days;and the absorption and utilization rate of nitrogen were reduced significantly as OsLSD2 over-expressed.The phenotype of transformed lines suggested the overexpression of OsLSD2 can significantly repress the growth and nitrogen translocation in rice.