CAJ | 학술논문

为了阐明不同 BT 型杂交粳稻组合间育性差异的原因，利用豫粳6号 A、9201A 及其相应保持系与恢复系 TR2604、闵120来配制遗传组合就 BT 型不育细胞质育性恢复遗传进行研究。根据9201A//9201B/闵120、9201A//9201B/TR2604及豫粳6号 A//豫粳6号 B/闵120三个群体中植株的育性分离，推断9201A、豫粳6号 A 的育性恢复均由1对恢复基因控制。基因定位结果表明，恢复系闵120中的恢复基因位于第10染色体上18772374-18967996bp 间约195 kb 物理区间内， TR2604中的恢复基因位于第10染色体18817631-18967996 bp 间约150 kb 物理区间内。根据已克隆 Rf1基因的信息可知两恢复系带有的恢复基因均为 Rf1a 。豫粳6号 A/TR2604//TR2604 BC1 F2群体中植株的 Rf1a 位点基因型与育性分析结果表明 TR2604对豫粳6号 A 育性恢复由 Rf1a 控制，豫粳6号 A/TR2604不育由双亲间的不亲和性造成。
위료천명불동 BT 형잡교갱도조합간육성차이적원인，이용예갱6호 A、9201A 급기상응보지계여회복계 TR2604、민120래배제유전조합취 BT 형불육세포질육성회복유전진행연구。근거9201A//9201B/민120、9201A//9201B/TR2604급예갱6호 A//예갱6호 B/민120삼개군체중식주적육성분리，추단9201A、예갱6호 A 적육성회복균유1대회복기인공제。기인정위결과표명，회복계민120중적회복기인위우제10염색체상18772374-18967996bp 간약195 kb 물리구간내， TR2604중적회복기인위우제10염색체18817631-18967996 bp 간약150 kb 물리구간내。근거이극륭 Rf1기인적신식가지량회복계대유적회복기인균위 Rf1a 。예갱6호 A/TR2604//TR2604 BC1 F2군체중식주적 Rf1a 위점기인형여육성분석결과표명 TR2604대예갱6호 A 육성회복유 Rf1a 공제，예갱6호 A/TR2604불육유쌍친간적불친화성조성。
In order to understand the genetic basis of spikelet fertility differences between BT-type three-line japonica hybrids,the CMS lines Yujing 6A,9201A and their corresponding maintainer lines,and the restorer lines Min 120, TR2604 were used to develop the genetic populations for studying the genetic characteristics of BT-type CMS in different hybrid combinations.According to fertility segregation of the plants from the populations of 9201A//9201B/Min 120,Yujing 6A//Yujing 6B/Min 120 and 9201A//9201B/TR2604,it was concluded that the fertility restoration of 9201A and Yujing 6A was controlled by one fertility restoration(Rf )gene.Then,the Rf genes in Min120,TR2604 were mapped between 18 772 374-18 967 996 bp with the physical distance of 1 95 kb and 18 81 7 63 1 -18 967 996 bp with the physical distance of 1 50 kb on chromosome 10,respectively,indicating that the Rf genes in Min 120, TR2604 might be Rf1a according to the mapping results of Rf1 in former studies.The fertility restoration of Yujing 6A in the population derived from Yujing 6A and TR2604 was also controlled by Rf1a identified by the genotype and phenotype of plants in Yujing 6A/TR2604//TR2604 BC1 F2 population,so the sterility of Yujing 6A/TR2604 was induced by hybrid incompatibility between the two parents.