CAJ | 학술논문

在 EMS 诱变的日本晴突变体库中，筛选到一个类病斑突变体.该突变体类病斑出现于分蘖始期，而后慢慢扩散至叶鞘、茎秆，最后至整个植株，属于扩散型类病斑突变体.相比野生型，突变体的 lmm4的株高明显降低，有效穗数减少，穗长缩短，结实率、每穗实粒数、千粒重均显著降低.遮光处理实验表明，突变体 lmm4类病斑的产生受自然光诱导.突变体lmm4的光合色素含量和净光合速率明显低于野生型.电子显微镜观察发现突变体类病斑部位叶肉细胞中的叶绿体结构发育异常.台盼蓝染色实验表明突变体类病斑部有大量的死亡细胞.二氨基联苯胺染色实验表明类病斑部位有过氧化氢沉积.稻瘟病抗性鉴定结果表明，与野生型相比，突变体 lmm4对 C7和 G1等稻瘟病菌生理小种的抗性明显增强.实时 PCR分析证明在突变体 lmm4中防卫反应相关基因 POC1、PAL 、PBZ1、PR1的表达量显著提高.遗传分析表明突变体表型符合单隐性核基因控制的遗传规律.lmm4定位在第5染色体着丝粒附近 Ind-4和 Ind-6之间大约235 kb 的区域内.
재 EMS 유변적일본청돌변체고중，사선도일개류병반돌변체.해돌변체류병반출현우분얼시기，이후만만확산지협초、경간，최후지정개식주，속우확산형류병반돌변체.상비야생형，돌변체적 lmm4적주고명현강저，유효수수감소，수장축단，결실솔、매수실립수、천립중균현저강저.차광처리실험표명，돌변체 lmm4류병반적산생수자연광유도.돌변체lmm4적광합색소함량화정광합속솔명현저우야생형.전자현미경관찰발현돌변체류병반부위협육세포중적협록체결구발육이상.태반람염색실험표명돌변체류병반부유대량적사망세포.이안기련분알염색실험표명류병반부위유과양화경침적.도온병항성감정결과표명，여야생형상비，돌변체 lmm4대 C7화 G1등도온병균생리소충적항성명현증강.실시 PCR분석증명재돌변체 lmm4중방위반응상관기인 POC1、PAL 、PBZ1、PR1적표체량현저제고.유전분석표명돌변체표형부합단은성핵기인공제적유전규률.lmm4정위재제5염색체착사립부근 Ind-4화 Ind-6지간대약235 kb 적구역내.
The lesion mimic mutant lmm4 was identified from an EMS-induced Nipponbare mutant library.Brown lesions were first observed on the tip of lmm4 leaves at the initial tillering stage,and spreaded gradually downward to the whole leaf blade and even the leaf sheath.Compared with the wild type,the chlorophyll and the net photosynthetic rate of lmm4 were significantly reduced.The plant height,panicle length,seed setting rate,grain weight were also decreased significantly.The shading assay showed that the lesions on lmm4 leaves were induced by natural light.The histological staining assay indicated that the lesions on lmm4 leaves were caused by programmed cell death and hydrogen peroxide accumulated in lmm4 leaves.The lmm4 displayed higher resistance to blast race C7 and G1 when compared with the wild type.In addition,pathogenesis-related gene POC1 ,PAL ,PBZ1 ,PR1 were found to be up-regulated in lmm4 .Genetic analysis suggested that the phenotype of lmm4 was controlled by a single recessive nuclear locus.Based a map-based cloning strategy,the lmm4 was narrowed down to a 235 kb region between Ind-4 and Ind-6 near the centromere of chromosome 5 .