CAJ | 학술논문

通过连锁分析和关联分析两种方法寻找与水稻芽期和幼苗前期耐盐性 QTL,并进一步挖掘优异等位变异及载体材料.连锁分析以高产优质水稻品种东农425为轮回亲本,耐盐性水稻品种长白10号为供体亲本构建 BC2 F2：3群体,利用137个 SSR 标记构建遗传连锁图谱,采用 ICIM 法对相对发芽率(RGR)、相对苗高(RSH)、相对根数(RRN)和相对根长(RRL)等4个与水稻芽期和幼苗前期耐盐性相关的性状进行 QTL 定位分析,共检测到11个 QTL.关联分析以341份粳稻种质组成的自然群体为研究材料,利用160个 SSR 标记进行群体基因型检测,采用 Tassel 2．1的 GLM 和 MLM 模型进行标记与性状的关联分析,共检测到22个关联位点,并验证了连锁分析中的5个 QTL,进一步鉴定得到9个优异等位基因.通过比较图谱发现,经验证的5个 QTL 中有4个与前人定位在同一或相邻的染色体区域,而 qRRL7在前人研究中未见报道.
통과련쇄분석화관련분석량충방법심조여수도아기화유묘전기내염성 QTL,병진일보알굴우이등위변이급재체재료.련쇄분석이고산우질수도품충동농425위륜회친본,내염성수도품충장백10호위공체친본구건 BC2 F2：3군체,이용137개 SSR 표기구건유전련쇄도보,채용 ICIM 법대상대발아솔(RGR)、상대묘고(RSH)、상대근수(RRN)화상대근장(RRL)등4개여수도아기화유묘전기내염성상관적성상진행 QTL 정위분석,공검측도11개 QTL.관련분석이341빈갱도충질조성적자연군체위연구재료,이용160개 SSR 표기진행군체기인형검측,채용 Tassel 2．1적 GLM 화 MLM 모형진행표기여성상적관련분석,공검측도22개관련위점,병험증료련쇄분석중적5개 QTL,진일보감정득도9개우이등위기인.통과비교도보발현,경험증적5개 QTL 중유4개여전인정위재동일혹상린적염색체구역,이 qRRL7재전인연구중미견보도.
QTL for salt tolerance at the germination and early seedling stage in rice were detected via linkage and association analyses.A BC2 F2 :3 population was constructed including 1 90 BC2 F3 lines,derived from a cross between Dongnong 425 (high-yielding rice variety with good grain quality,as the recurrent parent)and Changbai 10 (salt tolerant rice variety,as the donor parent).QTLs were detected for salt tolerance at the germination and early seedling stage including relative germination rate (RGR),relative seedling height (RSH ),relative root number (RRN), relative root length (RRL)were detected by using ICIM with a genetic linkage map constructed with 1 37 SSR markers. A total of 1 1 QTLs were identified.A panel of 341 japonica rice accessions from different geographical origins were used for both whole-genome association and targeted regional association mapping.The accessions were genotyped with 1 60 selected SSR markers,and then association analysis between SSR markers and traits were performed using TASSEL 2.1 GLM and MLM programs.A total of 22 significant marker-trait associations were identified within 18 markers, and 5 QTLs reported by linkage mapping were validated,and 9 novel alleles at these loci were mined from the set of japonica rice.By comparing the chromosomal positions of the 5 QTLs with those previously identified,four of them located in the same or near genome regions,qRRL7 was reported for the first time.