CAJ | 학술논문

为了挖掘水稻中的根系特异表达启动子，通过生物信息学分析和 RT-PCR 验证获得1个在根尖特异表达的水稻基因 Os04g24469.用分离出的该基因上游启动子，与 GUS 报告基因构建表达载体并转化到受体水稻日本晴中.通过对转基因后代中不同组织器官中的 GUS 活性检测，证明该启动子在水稻根尖特异性强烈表达.结合该根尖特异表达启动子，我们分析了一个在水稻地上部及地下部都组成表达的功能基因 OsSRG 对水稻根系发育的贡献.该基因的缺失导致水稻突变体srg 根系变短，而利用分离的根尖特异性 Os04g24469启动子在根尖驱动 GUS-OsSRG 的表达，能回复突变体根系生长，表明OsSRG 在根尖的表达足以影响水稻根系生长，而在地上部的表达与根系发育无关.本研究表明 Os04g24469启动子是一个水稻根尖特异表达启动子，利用该启动子可以控制目标基因在水稻根尖特异表达，可用于水稻根系基因功能的研究.
위료알굴수도중적근계특이표체계동자，통과생물신식학분석화 RT-PCR 험증획득1개재근첨특이표체적수도기인 Os04g24469.용분리출적해기인상유계동자，여 GUS 보고기인구건표체재체병전화도수체수도일본청중.통과대전기인후대중불동조직기관중적 GUS 활성검측，증명해계동자재수도근첨특이성강렬표체.결합해근첨특이표체계동자，아문분석료일개재수도지상부급지하부도조성표체적공능기인 OsSRG 대수도근계발육적공헌.해기인적결실도치수도돌변체srg 근계변단，이이용분리적근첨특이성 Os04g24469계동자재근첨구동 GUS-OsSRG 적표체，능회복돌변체근계생장，표명OsSRG 재근첨적표체족이영향수도근계생장，이재지상부적표체여근계발육무관.본연구표명 Os04g24469계동자시일개수도근첨특이표체계동자，이용해계동자가이공제목표기인재수도근첨특이표체，가용우수도근계기인공능적연구.
To obtain the root-specific promoters in rice,a root-specific gene Os04g24469 was selected by analyzing the public microarray data and further confirmed by RT-PCR.To determine the expression pattern of the novel promoter, the putative promoter was isolated and fused with the GUS reporter gene to transform into the rice variety Nipponbare. Subsequent GUS staining of the transgenic plants showed that the Os04g24469 promoter was only activated in rice root tip.Using this root tip-specific promoter,we characterized the function of a ubiquitously expressed gene OsSRG in root development.The absence of OsSRG led to a short root phenotype in the srg mutant,the root tip-specific expression of GUS-OsSRG in srg plants significantly reversed the root growth defect,indicating that the expression of OsSRG in root tip,not in shoot,is important for root development in rice.The present study showed that Os04g24469 promoter is highly active in the root tip and can be useful for the root tip-specific expression of target genes and gene function analysis in rice root.