水产科学
水產科學
수산과학
FISHERIES SCIENCE
2014年
6期
374-378
,共5页
曲焕韬%刘勇%郭文韬%肖衎%管敏%李莎%鲁雪报
麯煥韜%劉勇%郭文韜%肖衎%管敏%李莎%魯雪報
곡환도%류용%곽문도%초간%관민%리사%로설보
厚颌鲂%间氨基苯甲酸乙酯甲磺酸盐%麻醉%有效质量浓度
厚頜魴%間氨基苯甲痠乙酯甲磺痠鹽%痳醉%有效質量濃度
후합방%간안기분갑산을지갑광산염%마취%유효질량농도
Megalobrama pellegrini%MS-222%anaesthetia%effective dose
24~25℃,研究了间氨基苯甲酸乙酯甲磺酸盐对厚颌鲂幼鱼的麻醉效果。根据厚颌鲂幼鱼在麻醉和复苏过程的行为特征,将其麻醉过程分为5期,复苏过程分为4期。试验结果表明,厚颌鲂幼鱼的入麻时间随麻醉剂质量浓度的升高不断缩短(F=430.263,P<0.01),在该试验条件下,间氨基苯甲酸乙酯甲磺酸盐麻醉厚颌鲂幼鱼的有效质量浓度为70~120 mg/L ;麻醉过程中厚颌鲂幼鱼的呼吸频率先急剧升高(应激期~镇静期),然后缓慢下降(镇静期~深度麻醉期),直至过度麻醉停止呼吸(死亡期);深度麻醉状态下,试验鱼在空气中的暴露时间与复苏时间呈正相关(F=145.416,P<0.01),除短时间暴露的2个试验组(3 min和4 min)复苏时间差异不显著(P>0.05)外,其余各组差异均极显著(P<0.01);暴露时间在5 min以内,复苏率100%,超过5 min ,只有部分试验鱼可以复苏,当暴露时间达到12 m in时,厚颌鲂幼鱼全部死亡。
24~25℃,研究瞭間氨基苯甲痠乙酯甲磺痠鹽對厚頜魴幼魚的痳醉效果。根據厚頜魴幼魚在痳醉和複囌過程的行為特徵,將其痳醉過程分為5期,複囌過程分為4期。試驗結果錶明,厚頜魴幼魚的入痳時間隨痳醉劑質量濃度的升高不斷縮短(F=430.263,P<0.01),在該試驗條件下,間氨基苯甲痠乙酯甲磺痠鹽痳醉厚頜魴幼魚的有效質量濃度為70~120 mg/L ;痳醉過程中厚頜魴幼魚的呼吸頻率先急劇升高(應激期~鎮靜期),然後緩慢下降(鎮靜期~深度痳醉期),直至過度痳醉停止呼吸(死亡期);深度痳醉狀態下,試驗魚在空氣中的暴露時間與複囌時間呈正相關(F=145.416,P<0.01),除短時間暴露的2箇試驗組(3 min和4 min)複囌時間差異不顯著(P>0.05)外,其餘各組差異均極顯著(P<0.01);暴露時間在5 min以內,複囌率100%,超過5 min ,隻有部分試驗魚可以複囌,噹暴露時間達到12 m in時,厚頜魴幼魚全部死亡。
24~25℃,연구료간안기분갑산을지갑광산염대후합방유어적마취효과。근거후합방유어재마취화복소과정적행위특정,장기마취과정분위5기,복소과정분위4기。시험결과표명,후합방유어적입마시간수마취제질량농도적승고불단축단(F=430.263,P<0.01),재해시험조건하,간안기분갑산을지갑광산염마취후합방유어적유효질량농도위70~120 mg/L ;마취과정중후합방유어적호흡빈솔선급극승고(응격기~진정기),연후완만하강(진정기~심도마취기),직지과도마취정지호흡(사망기);심도마취상태하,시험어재공기중적폭로시간여복소시간정정상관(F=145.416,P<0.01),제단시간폭로적2개시험조(3 min화4 min)복소시간차이불현저(P>0.05)외,기여각조차이균겁현저(P<0.01);폭로시간재5 min이내,복소솔100%,초과5 min ,지유부분시험어가이복소,당폭로시간체도12 m in시,후합방유어전부사망。
The anaesthetic effect of MS‐222 on Megalobrama pellegrini juveniles was studied in water tanks in a laboratory at water temperature of 24—25 ℃ .In terms of the behavior characteristic of the fish , the anaesthetia was divided into five stages ,and the recovery process was divided into four stages .The fish was found to be anaesthetized more rapidly with increase in the concentration of MS‐222 (F=430 .263 ,P< 0 .01) ,with effective dose of 70—120 mg/L for anaesthetia . The fish showed rapid increase in respiratory frequency during the anaesthetic stage 1 and stage 2 first ,and then decline slowly from stage 2 to stage 4 ,until the fish stopped opercula moving in the stage of 5 due to excessive anaesthetia .There was a positive correlation between exposure time to air and recovery time for deeply anaesthetized fish (F=145 .416 ,P < 0 .01) ,significant difference(P<0 .01) among different exposure time groups except the two shorter exposure groups(3 min and 4 min) .The fish had recovery rate of 100% when the fish was exposed to MS‐222 within 5 min ,and only a few fish was recovered over 5 min .The fish came to death when the fish was exposed to MS‐222 for 12 min .