CAJ | 학술논문

以菠萝皮渣为原料，采用酸碱处理法从菠萝皮渣中提取菠萝皮渣纤维，主要对木聚糖酶酶解菠萝皮渣纤维制备低聚木糖的工艺进行研究。采用单因素试验法分析影响酶解效果的主要因素，利用正交试验优化酶解法的最佳工艺条件。结果表明，制备的菠萝皮渣纤维主要成分为粗纤维，约占90.09%，其中纤维素56.71%，半纤维素32.20%，酸不溶性木质素0.48%；木聚糖酶酶解菠萝皮渣粗纤维最优条件为反应温度50℃， pH值6，底物质量浓度60 mg/mL，酶用量1650 U，反应时间3 h，提取率为12.27%，低聚木糖平均聚合度DP为2.38。
이파라피사위원료，채용산감처리법종파라피사중제취파라피사섬유，주요대목취당매매해파라피사섬유제비저취목당적공예진행연구。채용단인소시험법분석영향매해효과적주요인소，이용정교시험우화매해법적최가공예조건。결과표명，제비적파라피사섬유주요성분위조섬유，약점90.09%，기중섬유소56.71%，반섬유소32.20%，산불용성목질소0.48%；목취당매매해파라피사조섬유최우조건위반응온도50℃， pH치6，저물질량농도60 mg/mL，매용량1650 U，반응시간3 h，제취솔위12.27%，저취목당평균취합도DP위2.38。
The pineapple peel cellulose is extracted by acid-alkali method from pineapple peel, then the production of xylooligosaccharides from pineapple peel cellulose with xylanase is mainly studied. The main factors of enzymatic hydrolysis are analyzed by single-factor experiment, and the optimal condition of enzyme hydrolysis is determined by using the orthogonal experimental based on single-factor experiment. The results show that crude fiber accounted about 90.09% in pineapple peel cellulose. The main constituents of the crude fiber are cellulose that account for 56.71%, and hemicellulose account for 32.20%, lignin account for 0.48%. The optimal conditions of enzyme hydrolysis are action temperature 50 ℃, pH value 6, substrate concentration 60 mg/mL, enzyme dosage 1 650 U, and time 3 h. The extraction rate is 12.27% under these conditions, and the average polymerization degree (DP) of the xylooligosaccharide is 2.38.