CAJ | 학술논문

目的：研究菊参的化学成分并通过药理实验寻找活性成分，为进一步开发利用该药用资源提供依据。方法：在药理活性筛选结果指导下，采用硅胶柱色谱、大孔树脂柱色谱、高效液相色谱等方法对其化学成分进行分离纯化，应用UV、IR、MS、NMR等分析技术对化合物的结构进行鉴定。结果：菊参正丁醇萃取部分能延长小鼠常压缺氧状态下的存活时间（P<0.05）。并从中分离得到了2个生物碱类化合物---1，2，3，4-四氢-β-咔啉-3-甲酸（玉）和腺嘌呤（域）。结论：化合物玉和域均为首次从菊参植物中分离获得。
목적：연구국삼적화학성분병통과약리실험심조활성성분，위진일보개발이용해약용자원제공의거。방법：재약리활성사선결과지도하，채용규효주색보、대공수지주색보、고효액상색보등방법대기화학성분진행분리순화，응용UV、IR、MS、NMR등분석기술대화합물적결구진행감정。결과：국삼정정순췌취부분능연장소서상압결양상태하적존활시간（P<0.05）。병종중분리득도료2개생물감류화합물---1，2，3，4-사경-β-잡람-3-갑산（옥）화선표령（역）。결론：화합물옥화역균위수차종국삼식물중분리획득。
This article was aimed to study the chemical constituents in Tragopogon porrifolius L. and their activities by pharmacological experiment in order to provide evidences in the further development of the usage of this medical resource. Under the guidance of pharmacological activities screening results, compounds were isolated by repeated silica gel, macroporous resin column chromatography and HPLC. Their structures were identified by means of UV, IR, MS, NMR and other chemical evidences. The results showed that T. porrifolius L. (i.e., n-butanol extraction part) can increase survival time of mice in the oxygen-lacking state (P < 0.05). Two compounds of biological alkaloids, which were identified as 1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid (Ⅰ) and adenine (II), were isolated. It was concluded that compound Ⅰ and II were obtained from T. porrifolius L. for the first time.