CAJ | 학술논문

目的：研究黄藤中黄藤素的提取和分离方法，进而以黄藤素为原料制备延胡索乙素。方法：采用L9（34）正交试验优化黄藤素的提取方法；采用大孔树脂层析技术研究黄藤素的分离方法；采用硼氢化钠氢化黄藤素的反应制备延胡索乙素。结果：10倍量（v/w）50%乙醇回流提取3次，每次2 h ，获得黄藤素的含量为36.40%，收率为3.17%；使用HPD-300大孔树脂吸附，纯化水、20%、60%乙醇溶液梯度洗脱分离，得到黄藤素含量为65.47%，收率为82.44%；氢化制得延胡索乙素含量为98.31%，收率为72.04%。结论：此方法可制得较高含量和收率的黄藤素，用此原料能制得较高含量和收率的延胡索乙素。
목적：연구황등중황등소적제취화분리방법，진이이황등소위원료제비연호색을소。방법：채용L9（34）정교시험우화황등소적제취방법；채용대공수지층석기술연구황등소적분리방법；채용붕경화납경화황등소적반응제비연호색을소。결과：10배량（v/w）50%을순회류제취3차，매차2 h ，획득황등소적함량위36.40%，수솔위3.17%；사용HPD-300대공수지흡부，순화수、20%、60%을순용액제도세탈분리，득도황등소함량위65.47%，수솔위82.44%；경화제득연호색을소함량위98.31%，수솔위72.04%。결론：차방법가제득교고함량화수솔적황등소，용차원료능제득교고함량화수솔적연호색을소。
This study was aimed to extract and separate fibrauretine from Chinese herbal medicine Fibraurea recisa Pierre. and to prepare tetrahydropulmatine with fibrauretine. Extraction method of fibrauretine were optimized by the L9(34) orthogonal arrays as experimental designs. Then, elution chromatography method was studied with the macroporous adsorption resin chromatography technology. At last, tetrahydropulmatine was prepared by the reaction of sodium borohydride hydrogenating fibrauretine. The results showed that the content and yield of obtained fibrauretine were 36.40% and 3.17%, respectively. The reflux exacted methods of fibrauretine were with 10 times amount (v/w) of 50%ethanol solution for 2 hours and 3 times. Then, HPD-300 macroporous resin was used to absorb fibrauretine, and purify water. The 20% and 60% ethanol solution was used for elution. The content and yield of obtained fibrauretine were 65.47% and 82.44%, respectively. And the content and yield of obtained tetrahydropulmatine were 98.31% and 72.04%, respectively. It was concluded that the method can obtain fibrauretine with relative high content and yield. Using this material can obtain tetrahydrofibrauretine with relative high content and yield.