世界科学技术-中医药现代化
世界科學技術-中醫藥現代化
세계과학기술-중의약현대화
WORLD SCIENCE AND TECHNOLOGY-MODERNIZATION OF TRADITIONAL CHINESE MEDICINE
2014年
6期
1391-1395
,共5页
何刚%卿光明%李敏%杨行军%罗远鸿%任敏
何剛%卿光明%李敏%楊行軍%囉遠鴻%任敏
하강%경광명%리민%양행군%라원홍%임민
鱼腥草%GC/MS%4-萜品醇%α-松油醇%乙酸龙脑酯%甲基正壬酮
魚腥草%GC/MS%4-萜品醇%α-鬆油醇%乙痠龍腦酯%甲基正壬酮
어성초%GC/MS%4-첩품순%α-송유순%을산용뇌지%갑기정임동
Houttuynia cordata%GC/MS%4-terpineol%α-terpineol%bornyl acetate%methyl-n-nonylketone
目的：首次建立气相色谱-质谱联用法（GC-MS）对鱼腥草鲜品挥发油中4-萜品醇、α-松油醇、乙酸龙脑酯和甲基正壬酮4种成分的含量测定方法，为鱼腥草药材质量控制提供实验依据。方法：以内标物质为正十一碳烯，采用GC/MS法测定鲜品鱼腥草挥发油中4种成分的含量。气相色谱条件：程序升温条件：70℃持续5 min，5℃·min-1升温，140℃持续5 min，10℃·min-1升温，250℃持续10 min。进样口温度为230℃，载气为高纯He，流速为1.0 mL·min-1；分流比为10：1；进样量为1μL。质谱条件：检测器为MS， EI为70EV，四极杆温度为150℃，离子源温度为230℃。结果：4-萜品醇、α-松油醇、乙酸龙脑酯和甲基正壬酮分别在6.492~129.84μg·mL-1，1.097~21.944μg·mL-1，12.128~242.56μg·mL-1，84.76~169.52μg·mL-1范围内线性关系良好。平均回收率分别为81.2%，80.6%，88.3%，84.6%；RSD分别为1.5%，2.3%，1.1%，0.7%。结论：该法简便、灵敏、易操作，采用同时测定多指标成分评价鱼腥草质量，能有效的反映鱼腥草质量，可用于鲜鱼腥草药材的质量评价。
目的：首次建立氣相色譜-質譜聯用法（GC-MS）對魚腥草鮮品揮髮油中4-萜品醇、α-鬆油醇、乙痠龍腦酯和甲基正壬酮4種成分的含量測定方法，為魚腥草藥材質量控製提供實驗依據。方法：以內標物質為正十一碳烯，採用GC/MS法測定鮮品魚腥草揮髮油中4種成分的含量。氣相色譜條件：程序升溫條件：70℃持續5 min，5℃·min-1升溫，140℃持續5 min，10℃·min-1升溫，250℃持續10 min。進樣口溫度為230℃，載氣為高純He，流速為1.0 mL·min-1；分流比為10：1；進樣量為1μL。質譜條件：檢測器為MS， EI為70EV，四極桿溫度為150℃，離子源溫度為230℃。結果：4-萜品醇、α-鬆油醇、乙痠龍腦酯和甲基正壬酮分彆在6.492~129.84μg·mL-1，1.097~21.944μg·mL-1，12.128~242.56μg·mL-1，84.76~169.52μg·mL-1範圍內線性關繫良好。平均迴收率分彆為81.2%，80.6%，88.3%，84.6%；RSD分彆為1.5%，2.3%，1.1%，0.7%。結論：該法簡便、靈敏、易操作，採用同時測定多指標成分評價魚腥草質量，能有效的反映魚腥草質量，可用于鮮魚腥草藥材的質量評價。
목적：수차건립기상색보-질보련용법（GC-MS）대어성초선품휘발유중4-첩품순、α-송유순、을산용뇌지화갑기정임동4충성분적함량측정방법，위어성초약재질량공제제공실험의거。방법：이내표물질위정십일탄희，채용GC/MS법측정선품어성초휘발유중4충성분적함량。기상색보조건：정서승온조건：70℃지속5 min，5℃·min-1승온，140℃지속5 min，10℃·min-1승온，250℃지속10 min。진양구온도위230℃，재기위고순He，류속위1.0 mL·min-1；분류비위10：1；진양량위1μL。질보조건：검측기위MS， EI위70EV，사겁간온도위150℃，리자원온도위230℃。결과：4-첩품순、α-송유순、을산용뇌지화갑기정임동분별재6.492~129.84μg·mL-1，1.097~21.944μg·mL-1，12.128~242.56μg·mL-1，84.76~169.52μg·mL-1범위내선성관계량호。평균회수솔분별위81.2%，80.6%，88.3%，84.6%；RSD분별위1.5%，2.3%，1.1%，0.7%。결론：해법간편、령민、역조작，채용동시측정다지표성분평개어성초질량，능유효적반영어성초질량，가용우선어성초약재적질량평개。
This study was aimed to establish a method for assay of 4-terpineol, α-terpineol, bornyl acetate and methyl-n-nonylketone in Houttuynia cordata with gas chromatography-mass spectrometry (GS/MS) for the first time in order to provide experimental evidences for its quality control. Undecylen was analyzed quantitatively. GC/MS method was used in the content determination of 4 kinds of components in Houttuynia cordata. GC conditions were that the temperature was firstly increased to 70oC for 5 min, and then 5oC·min-1 to 140oC for 5 min. Then, the temperature was increased by 10oC·min-1 to 250oC for 10 min. The temperature of the injection port was 230oC. The carrier gas was high purity helium. The flow rate was 1.0 mL·min-1. The split ratio was 10:1. The sample quantity was 1 μl. MS conditions were that the detector was MS, EI was 70ev, the temperature of four poles was 150oC, the temperature of ion source was 230oC. The results showed that there was good linearity of four components, which were 4-terpineol, α-terpineol, bornyl acetate and methyl-n-nonylketone obtained within the ranges of 6.492~129.84μg·min-1, 1.097~21.944 μg·min-1, 12.128~242.56 μg·min-1, and 84.76~169.52 μg·min-1, respectively. The average recoveries of the four components were 81.2%, 80.6%, 88.3%, and 84.6%, respectively. The RSDs were 1.5%, 2.3%, 1.1%, and 0.7%, respectively. It was concluded that the method was simple, sensitive, and easy to operate. The simultaneous measurement of multiple indexes on the component for quality evaluation of Houttuynia cordata can effectively reflect the quality of Houttuynia cordata. It can also be used in the quality assessment of fresh Houttuynia c ordate .
