CAJ | 학술논문

目的：观察补气活血药对急性心肌梗死模型大鼠梗死心肌边缘区新生微血管数目以及基质细胞衍生因子-1（SDF-1）、趋化因子受体（CXCR4）蛋白及mRNA表达的影响。方法：建立大鼠急性心肌梗死动物模型，采用免疫组化SP法检测心肌组织中VIII因子相关抗原（vWF）蛋白的表达，计数微血管数目（MVC）；用Western Blot和Real-Time PCR技术检测各组大鼠梗死心肌边缘区域的SDF-1因子和其特异性受体CXCR4蛋白和基因表达情况。结果：假手术组、模型组、各给药组大鼠梗死心肌边缘区域均可见vWF因子标记染色的新生微血管，假手术组大鼠心肌中新生微血管不明显，模型组大鼠梗死心肌边缘区域可见到少量新生成的微血管，各给药组大鼠梗死心肌边缘区域可见较多新生微血管。模型组与假手术组比较差异有统计学意义（P约0.05）；各给药组与模型组相比较差异有统计学意义（P约0.05或P约0.01）。与假手术组比较，模型组大鼠心肌中SDF-1、CXCR4蛋白和mRNA表达明显增多（P约0.05或P约0.01）；与模型组比较，各给药组SDF-1、CXCR4蛋白和mRNA表达均明显增多（P约0.05或P约0.01）。结论：丹参、丹参黄芪药对等药物可以促进血管的生成，推测这些药物促进血管生成的机制可能是通过促进SDF-1、CXCR4蛋白及mR原NA表达水平，从而增加SDF-1、CXCR4的含量以促进新生血管的生成。
목적：관찰보기활혈약대급성심기경사모형대서경사심기변연구신생미혈관수목이급기질세포연생인자-1（SDF-1）、추화인자수체（CXCR4）단백급mRNA표체적영향。방법：건립대서급성심기경사동물모형，채용면역조화SP법검측심기조직중VIII인자상관항원（vWF）단백적표체，계수미혈관수목（MVC）；용Western Blot화Real-Time PCR기술검측각조대서경사심기변연구역적SDF-1인자화기특이성수체CXCR4단백화기인표체정황。결과：가수술조、모형조、각급약조대서경사심기변연구역균가견vWF인자표기염색적신생미혈관，가수술조대서심기중신생미혈관불명현，모형조대서경사심기변연구역가견도소량신생성적미혈관，각급약조대서경사심기변연구역가견교다신생미혈관。모형조여가수술조비교차이유통계학의의（P약0.05）；각급약조여모형조상비교차이유통계학의의（P약0.05혹P약0.01）。여가수술조비교，모형조대서심기중SDF-1、CXCR4단백화mRNA표체명현증다（P약0.05혹P약0.01）；여모형조비교，각급약조SDF-1、CXCR4단백화mRNA표체균명현증다（P약0.05혹P약0.01）。결론：단삼、단삼황기약대등약물가이촉진혈관적생성，추측저사약물촉진혈관생성적궤제가능시통과촉진SDF-1、CXCR4단백급mR원NA표체수평，종이증가SDF-1、CXCR4적함량이촉진신생혈관적생성。
This study was aimed to observe the influence of qi-reinforcing and blood-activating(QRBA) herbs onan-giogenesis of the myocardial microvascular, SDF-1 and CXCR4 protein expression as well as mRNA expression in the infarcted myocardium edge area of acute myocardial infarction (AMI) ratmodel. The AMI rat model was estab-lished. The immunohistochemical staining method was used in the detection of vWF protein expression in the myocar-dial tissues. The MVC account was recorded. The SDF-1 factor and its specific receptor factor CXCR4 were detected by the western blot and realtime-PCR technique in the infarcted myocardium edge area of rats from each group. The results showed that in the new generated microvessels which were staining marked by the vWF factor can be seen in infarcted myocardium edge area of rats from the sham-operated group, model group, each medication group. The new generated microvessels in the myocardium of rats in the sham-operated group were not obvious. Small amount of new generated microvessels can be seen in rats from the model group. More new generated microvessels can be seen in rats from each medication group. The comparison between the model group and the sham-operated group showed sta-tistical difference (P<0.05). The comparison between each medication group and the model group showed statistical difference(P<0.05 or P<0.01). Compared with the sham-operated group, SDF-1, CXCR4 and mRNA expression were obviously increased in the myocardium of rats in the model group (P<0.05 or P<0.01). Compared with the model group, SDF-1, CXCR4 protein and mRNA expression were obviously increased in the myocardium of rats from each medication group (P<0.05 or P<0.01). It was concluded that herbs such as Salvia, couplet herbs of Salvia and Astra-galushad stimulation effectonangiogenesis. Mechanism of these drugs in angiogenesismay be through the promotion of SDF-1 and CXCR4 protein as well as mRNA express.