大众科技
大衆科技
대음과기
DAZHONG KEJI
2014年
6期
98-100
,共3页
米曲霉%β-葡萄糖苷酶%优化%响应面
米麯黴%β-葡萄糖苷酶%優化%響應麵
미곡매%β-포도당감매%우화%향응면
Aspergillusoryzae%β-glucosidase%optimization%response surface methodology
文章研究旨在优化前期筛选到的米曲霉HML366产β-葡萄糖苷酶的培养基条件。通过单因素实验和响应面设计对米曲霉HML366产β-葡萄糖苷酶的培养基条件进行了优化组合，确定最佳的发酵培养基配方为：甘蔗渣3.49%，豆饼粉0.375%，KH2PO40.255%。在此培养条件下得到β-葡萄糖苷酶酶活力达92.64 U/g，是未优化前酶活71.12 U/g的1.30倍。
文章研究旨在優化前期篩選到的米麯黴HML366產β-葡萄糖苷酶的培養基條件。通過單因素實驗和響應麵設計對米麯黴HML366產β-葡萄糖苷酶的培養基條件進行瞭優化組閤，確定最佳的髮酵培養基配方為：甘蔗渣3.49%，豆餅粉0.375%，KH2PO40.255%。在此培養條件下得到β-葡萄糖苷酶酶活力達92.64 U/g，是未優化前酶活71.12 U/g的1.30倍。
문장연구지재우화전기사선도적미곡매HML366산β-포도당감매적배양기조건。통과단인소실험화향응면설계대미곡매HML366산β-포도당감매적배양기조건진행료우화조합，학정최가적발효배양기배방위：감자사3.49%，두병분0.375%，KH2PO40.255%。재차배양조건하득도β-포도당감매매활력체92.64 U/g，시미우화전매활71.12 U/g적1.30배。
The study was to optimize the medium conditions ofβ-glucosidase production by preliminary screening Aspergillusoryzae HML366. Optimization of fermenting culture medium for producingβ-glucosidase by AspergillusoryzaeHML366 with single factor test and response surface methodology, the optimum components of mediums consisted of bagasse 3.49%, bean cake powder 0.375%, KH2PO4 0.255%. Under this condition theβ-glucosidase activity was up to 92.64U/g, it was 1.30 times compared with the activity 71.12 U/g before optimized.
