CAJ | 학술논문

目的：明确阿维 A 对系统性硬皮病成纤维细胞胶原合成及 I 型前胶原 mRNA 表达的影响。方法：原代培养系统性硬皮病(SSc)患者皮肤成纤维细胞(FB)，使用不同浓度的阿维 A(10-5、10-6、10-7、10-8 mol/ L)作用48 h，用羟脯氨酸法测定胶原的质量浓度，RT-PCR 法 I 型前胶原 mRNA 的表达。结果：阿维 A 实验组 FB 胶原合成及 I 型前胶原 mRNA 的表达较对照组减少，且均与阿维 A 呈浓度依赖性关系。结论：阿维 A 可能通过抑制 FB I 型前胶原基因的表达减少胶原合成。
목적：명학아유 A 대계통성경피병성섬유세포효원합성급 I 형전효원 mRNA 표체적영향。방법：원대배양계통성경피병(SSc)환자피부성섬유세포(FB)，사용불동농도적아유 A(10-5、10-6、10-7、10-8 mol/ L)작용48 h，용간포안산법측정효원적질량농도，RT-PCR 법 I 형전효원 mRNA 적표체。결과：아유 A 실험조 FB 효원합성급 I 형전효원 mRNA 적표체교대조조감소，차균여아유 A 정농도의뢰성관계。결론：아유 A 가능통과억제 FB I 형전효원기인적표체감소효원합성。
To determine the effect of acitretin on the collagen synthesis and the mRNA expression of proα1 (I) collagen in cultured systemic sclerosis fibroblasts(SSCFB). Methods: SSCFB were incubated with acitretin (10-5、10-6、10-7、10-8 mol/ L) for 48 hours. Hydroproline and RT-PCR were used to measure the content of collagen and the mRNA expression of proα1 (I) collagen. Results: The content of collagen and the mRNA expression of proα1 (I) collagen were lower than in acitretin group than in the control group, which were in a concentration-dependent manner. Conclusion: Collagen synthesis can be inhibited by acitretin through suppressing proα1 (I) collagen mRNA expression of SSc Fb.