中国麻风皮肤病杂志
中國痳風皮膚病雜誌
중국마풍피부병잡지
CHINA JOURNAL OF LEPROSY AND SKIN DISEASES
2014年
7期
390-393
,共4页
邓蕙妍%高爱莉%李振洁%张倩雯%万苗坚%朱慧兰
鄧蕙妍%高愛莉%李振潔%張倩雯%萬苗堅%硃慧蘭
산혜연%고애리%리진길%장천문%만묘견%주혜란
姜黄素%HaCaT细胞%急性光损伤%核转录相关因子Nrf2
薑黃素%HaCaT細胞%急性光損傷%覈轉錄相關因子Nrf2
강황소%HaCaT세포%급성광손상%핵전록상관인자Nrf2
curcumin%HaCaT cell%acute light damage%NF-E2-related factor 2
目的：明确姜黄素对急性紫外线损伤的HaCaT细胞内Nrf2核转位的影响。方法：1μM、2.5μM、5μM姜黄素与细胞共培养，UVA、UVB照射后Western blot法检测各实验组HaCaT细胞内Nrf2核转位情况。结果：姜黄素预处理后照射UVA和UVB，Nrf2胞质蛋白的浓度随姜黄素的浓度升高而下降，胞核蛋白浓度则随其升高而升高( P<0.05)。结论：姜黄素对急性紫外线损伤的HaCaT细胞内Nrf2核转位有激活作用。
目的：明確薑黃素對急性紫外線損傷的HaCaT細胞內Nrf2覈轉位的影響。方法：1μM、2.5μM、5μM薑黃素與細胞共培養，UVA、UVB照射後Western blot法檢測各實驗組HaCaT細胞內Nrf2覈轉位情況。結果：薑黃素預處理後照射UVA和UVB，Nrf2胞質蛋白的濃度隨薑黃素的濃度升高而下降，胞覈蛋白濃度則隨其升高而升高( P<0.05)。結論：薑黃素對急性紫外線損傷的HaCaT細胞內Nrf2覈轉位有激活作用。
목적：명학강황소대급성자외선손상적HaCaT세포내Nrf2핵전위적영향。방법：1μM、2.5μM、5μM강황소여세포공배양，UVA、UVB조사후Western blot법검측각실험조HaCaT세포내Nrf2핵전위정황。결과：강황소예처리후조사UVA화UVB，Nrf2포질단백적농도수강황소적농도승고이하강，포핵단백농도칙수기승고이승고( P<0.05)。결론：강황소대급성자외선손상적HaCaT세포내Nrf2핵전위유격활작용。
Objective:To determine the effect of curcumin on the activation of Nrf2 in HaCaT cells after acute light damage. Methods:1μM, 2.5μM and 5μM curcumin were cultured with HaCaT cells to establish acute light damage induced by UV irradiation. Western blot assay was used to analyze the nuclear translocation of Nrf2. Results:After UV irradiation the concentration of Nrf2 cytoplasmic protein was increased as the concentration of curcumin decreased, but the concentration of the nuclear protein was increased as the concentration of curcumin increased ( P<0.05) . Conclusion:The curcumin can cause HaCaT cell nuclear translocation of Nrf2 after acute UV damage.
