中国麻风皮肤病杂志
中國痳風皮膚病雜誌
중국마풍피부병잡지
CHINA JOURNAL OF LEPROSY AND SKIN DISEASES
2014年
7期
387-389
,共3页
牟宽厚%周艳%韩丹%穆欣
牟寬厚%週豔%韓丹%穆訢
모관후%주염%한단%목흔
苦参碱%HaCaT 细胞%实时定量 PCR%Bcl-2/ Bax%Fas/ FasL
苦參堿%HaCaT 細胞%實時定量 PCR%Bcl-2/ Bax%Fas/ FasL
고삼감%HaCaT 세포%실시정량 PCR%Bcl-2/ Bax%Fas/ FasL
marine%HaCaT cells%Real-time quantitative PCR%Bcl-2/ Bax%Fas/ FasL
目的:明确苦参碱对 HaCaT 细胞 Bcl-2/ Bax 和 Fas/ FasL 表达的影响。方法:体外培养HaCaT 细胞,选择第二代细胞对数生长期 HaCaT 细胞作为研究对象,将细胞随机分为4组:苦参碱2 mg/ mL、10 mg/ mL 和50 mg/ mL 3组及对照组(加入相同体积的0.9%盐水),孵育48 h 后,MTT 法测定各浓度下细胞增殖,RT-PCR 检测 Bcl-2/ Bax 和 Fas/ FasL 的表达。结果:与对照组相比,当苦参碱浓度为2 mg/ mL 时,HaCaT 细胞增殖活性无明显变化;Bcl -2、Bax、Fas、FasL 表达也无明显变化( P>0.05)。当苦参碱浓度为10 mg/ mL 时 HaCaT 细胞增殖活性较对照组明显下降(P<0.001);Bcl-2表达明显下降,Bax 表达上升(P<0.001);Fas、FasL 表达上升(P<0.05)。当苦参碱浓度为50 mg/ mL 时,MTT法检测 HaCaT 细胞增殖明显抑制(P<0.001);同时 Bcl-2、 Bax、Fas 和 FasL 的变化与10 mg/ mL 时相似(P>0.05)。结论:苦参碱能够调控上皮细胞致炎因子的表达,抑制细胞的增殖。
目的:明確苦參堿對 HaCaT 細胞 Bcl-2/ Bax 和 Fas/ FasL 錶達的影響。方法:體外培養HaCaT 細胞,選擇第二代細胞對數生長期 HaCaT 細胞作為研究對象,將細胞隨機分為4組:苦參堿2 mg/ mL、10 mg/ mL 和50 mg/ mL 3組及對照組(加入相同體積的0.9%鹽水),孵育48 h 後,MTT 法測定各濃度下細胞增殖,RT-PCR 檢測 Bcl-2/ Bax 和 Fas/ FasL 的錶達。結果:與對照組相比,噹苦參堿濃度為2 mg/ mL 時,HaCaT 細胞增殖活性無明顯變化;Bcl -2、Bax、Fas、FasL 錶達也無明顯變化( P>0.05)。噹苦參堿濃度為10 mg/ mL 時 HaCaT 細胞增殖活性較對照組明顯下降(P<0.001);Bcl-2錶達明顯下降,Bax 錶達上升(P<0.001);Fas、FasL 錶達上升(P<0.05)。噹苦參堿濃度為50 mg/ mL 時,MTT法檢測 HaCaT 細胞增殖明顯抑製(P<0.001);同時 Bcl-2、 Bax、Fas 和 FasL 的變化與10 mg/ mL 時相似(P>0.05)。結論:苦參堿能夠調控上皮細胞緻炎因子的錶達,抑製細胞的增殖。
목적:명학고삼감대 HaCaT 세포 Bcl-2/ Bax 화 Fas/ FasL 표체적영향。방법:체외배양HaCaT 세포,선택제이대세포대수생장기 HaCaT 세포작위연구대상,장세포수궤분위4조:고삼감2 mg/ mL、10 mg/ mL 화50 mg/ mL 3조급대조조(가입상동체적적0.9%염수),부육48 h 후,MTT 법측정각농도하세포증식,RT-PCR 검측 Bcl-2/ Bax 화 Fas/ FasL 적표체。결과:여대조조상비,당고삼감농도위2 mg/ mL 시,HaCaT 세포증식활성무명현변화;Bcl -2、Bax、Fas、FasL 표체야무명현변화( P>0.05)。당고삼감농도위10 mg/ mL 시 HaCaT 세포증식활성교대조조명현하강(P<0.001);Bcl-2표체명현하강,Bax 표체상승(P<0.001);Fas、FasL 표체상승(P<0.05)。당고삼감농도위50 mg/ mL 시,MTT법검측 HaCaT 세포증식명현억제(P<0.001);동시 Bcl-2、 Bax、Fas 화 FasL 적변화여10 mg/ mL 시상사(P>0.05)。결론:고삼감능구조공상피세포치염인자적표체,억제세포적증식。
To determine the effect of matrine on Bcl-2/ Bax and Fas/ FasL in keratinocytes in vitro. Methods: Second generation cultured HaCaT cells (logarithmic phase cells) were selected and divided into 4 groups:3 matrine groups (2 mg/ mL, 10 mg/ mL and 50 mg/ mL were used in each group) and the control group (0.9% Natrii Chloride). After 48-hour culture, the proliferation of HaCaT were detected by MTT and the levels of Bcl-2/ Bax and Fas/ FasL were measured by RT-PCR. Results: The viability of HaCaT cells was similar in 2 mg/ mL matrine group and control group (P>0.05). In 10 mg/ mL matrine group the proliferation of the cells was significantly decreased (P<0.001) and the Bcl-2 expression was remarkably reduced (P<0.001), while the expression of Bax, Fas and FasL was significantly increased (P<0.01 and P<0.05, respectively). When the concentration of matrine was increased to 50 mL, the viability and the expression of Bcl-2, Bax, Fas and FasL was similar to the results when 10 mL matrine was used. Conclusion: Matrine can inhibit HaCaT cells proliferation (at 10 mg/ mL or more) and may adjust expression of Bcl-2/ Bax and Fas/FasL in HaCaT cells.