中国药理学通报
中國藥理學通報
중국약이학통보
CHINESE PHARMACOLOGICAL BULLETIN
2014年
7期
1026-1030
,共5页
崔悦%赵佳%王晔%孙丹丹%张莹%刘洋%赵小亮%牛晓红%张美玉%王丹巧%高天乐%徐晓军
崔悅%趙佳%王曄%孫丹丹%張瑩%劉洋%趙小亮%牛曉紅%張美玉%王丹巧%高天樂%徐曉軍
최열%조가%왕엽%손단단%장형%류양%조소량%우효홍%장미옥%왕단교%고천악%서효군
光化学诱导%三叉神经痛%纹状体细胞外液%Tac1 mRNA%谷氨酸%微透析
光化學誘導%三扠神經痛%紋狀體細胞外液%Tac1 mRNA%穀氨痠%微透析
광화학유도%삼차신경통%문상체세포외액%Tac1 mRNA%곡안산%미투석
photochemical injury%trigeminal neuralgia%striatal extracellular fluid%Tac1mRNA%glutamate%microdialysis
目的：观察光化学损伤诱导的三叉神经痛（PITN ）大鼠模型的行为学、周围和中枢神经系统疼痛相关递质／调质的变化及对药物的反应，为TN的基础研究提供可用的动物模型。方法将适应性刺激筛选合格的SD ♂大鼠随机分为假手术对照组、TN 模型组、TN 加巴喷丁组（100 mg · kg－1），模型以赤藓红B静脉注射加激光照射方法诱发。采用Von Frey hairs测量大鼠机械痛阈值，评价其行为学变化；荧光定量PCR技术检测三叉神经节内Tac1 mRNA表达量，脑内微透析方法采集脑纹状体细胞外液，高效液相－荧光法检测其中谷氨酸（Glu ）的水平。结果大鼠三叉神经支配的颜面部位机械痛阈平均值于造模d7后稳定在4 g以下，模型成功率为63％；造模前后自身对照，痛阈值由26 g降低为（1.60±1.74）g（P＜0.01）；术后 d 10模型组（1.63±
目的：觀察光化學損傷誘導的三扠神經痛（PITN ）大鼠模型的行為學、週圍和中樞神經繫統疼痛相關遞質／調質的變化及對藥物的反應，為TN的基礎研究提供可用的動物模型。方法將適應性刺激篩選閤格的SD ♂大鼠隨機分為假手術對照組、TN 模型組、TN 加巴噴丁組（100 mg · kg－1），模型以赤蘚紅B靜脈註射加激光照射方法誘髮。採用Von Frey hairs測量大鼠機械痛閾值，評價其行為學變化；熒光定量PCR技術檢測三扠神經節內Tac1 mRNA錶達量，腦內微透析方法採集腦紋狀體細胞外液，高效液相－熒光法檢測其中穀氨痠（Glu ）的水平。結果大鼠三扠神經支配的顏麵部位機械痛閾平均值于造模d7後穩定在4 g以下，模型成功率為63％；造模前後自身對照，痛閾值由26 g降低為（1.60±1.74）g（P＜0.01）；術後 d 10模型組（1.63±
목적：관찰광화학손상유도적삼차신경통（PITN ）대서모형적행위학、주위화중추신경계통동통상관체질／조질적변화급대약물적반응，위TN적기출연구제공가용적동물모형。방법장괄응성자격사선합격적SD ♂대서수궤분위가수술대조조、TN 모형조、TN 가파분정조（100 mg · kg－1），모형이적선홍B정맥주사가격광조사방법유발。채용Von Frey hairs측량대서궤계통역치，평개기행위학변화；형광정량PCR기술검측삼차신경절내Tac1 mRNA표체량，뇌내미투석방법채집뇌문상체세포외액，고효액상－형광법검측기중곡안산（Glu ）적수평。결과대서삼차신경지배적안면부위궤계통역평균치우조모d7후은정재4 g이하，모형성공솔위63％；조모전후자신대조，통역치유26 g강저위（1.60±1.74）g（P＜0.01）；술후 d 10모형조（1.63±
Aim To investigate the behavioral changes of the pain related neuromodulation and neurotransmission in peripheral and central nervous systems in rats with trigeminal neuralgia (TN)and provide a disease relevant animal model for mecha-nism study of TN.Methods The male SD rats were randomly divided into sham operation group and TN surgical group.The latter group was further divided into model group and gabapentin group (100 mg · kg-1 ). TN was induced by intravenous erythrosine B injection and laser irradiation.The pain behavior of rats was evaluated using mechanical pain threshold measured with Von Frey hairs.Fluorescence quantitative PCR technique was deployed to study the change of Tac1 mRNA expressions in trigeminal ganglia.Utilizing microdialysis technique followed by high performance liquid chromatography fluorescence detection (HPLC-FLD),the extracellular striatum fluid was collected and glutamate(Glu)concentration was determined.Results In the model group,the average mechanical pain threshold in facial ar-ea innervated by the trigeminal nerve remained below 4g after 7 days post surgery.The mechanical threshold of the model group (1.63 ±1.27)g was significantly lower (P<0.01)than the control group (24.17 ±4.49)g on day10 post surgery.In gen-eral,the mechanical withdraw threshold was decreased from the preoperative value of 26g to the postoperative value of (1.60 ± 1.74)g (P<0.01),and maintained stable at (0.71 ±1.24) g during the whole dynamic monitoring period from day7 to day60.The successful rate of this model was 63%.After sur-gery,Tac1 mRNA expression in trigeminal ganglia and extracel-lular Glu levels in striatum were significantly up-regulated (P<0.05 ) in the model group. Animals receiving Gabapentin showed significant improvement in pain symptoms,as well as re-ductions of Tac1 mRNA expression in trigeminal ganglia and ex-tracellular Glu concentration in striatum (P<0.05 ).Conclu-sions The above described photochemically induced TN rat model can partially mimic the clinical TN symptoms and its pathophysiology.Considering its overall high stability,it is very likely that this model could be used in preclinical mechanism study or drug screening of TN.
