CAJ | 학술논문

目的：观察离体大鼠急性缺血心肌内源性硫化氢／胱硫醚-γ-裂解酶（H2 S／CSE ）体系的动态变化，研究H2 S 对急性心肌缺血损伤大鼠心脏功能，心肌组织H2 S／CSE 及心肌梗死体积的影响。方法应用Langendorff心脏灌注系统建立急性心肌缺血损伤模型。分别于相应时间点取材，取材前应用 Powerlab／8s 多导生理仪记录各组大鼠左室发展压（LVDP）、左室压力变化速率（±dp／dtmax ）；测定冠脉流量（CF）；检测心肌组织中H2 S含量及CSE 活性；以Evan′s蓝和TTC双染法检测心肌梗死体积。结果①与相应假手术组比较，缺血30 min、1、2、3、4 h组LVDP、±dp／dtmax、CF 明显降低（P＜0．01）。缺血30 min组H2S含量和CSE活性无明显变化，心肌组织无明显梗死灶，缺血1、2、3、4 h组H2 S含量和CSE活性均明显降低（P＜0．05或P＜0．01）。缺血1 h组可见明显梗死灶（P＜0．01），缺血2、3、4 h组梗死体积明显增大（P＜0．01）；②与缺血4 h组比较，NaHS低、中、高剂量组 LVDP、±dp／dtmax、CF 明显升高（P ＜0．05或 P ＜0.01），H2S含量和CSE活性均升高（P＜0.05或P＜0.01）， NaHS中、高剂量组心肌梗死体积明显减小（P＜0.01）。结论 H2 S与CSE参与了离体大鼠急性心肌缺血损伤。NaHS可增强急性缺血损伤心肌CSE活性，增加H2 S含量，使心肌梗死体积缩小，提示H2 S可改善急性心肌缺血损伤。
목적：관찰리체대서급성결혈심기내원성류화경／광류미-γ-렬해매（H2 S／CSE ）체계적동태변화，연구H2 S 대급성심기결혈손상대서심장공능，심기조직H2 S／CSE 급심기경사체적적영향。방법응용Langendorff심장관주계통건립급성심기결혈손상모형。분별우상응시간점취재，취재전응용 Powerlab／8s 다도생리의기록각조대서좌실발전압（LVDP）、좌실압력변화속솔（±dp／dtmax ）；측정관맥류량（CF）；검측심기조직중H2 S함량급CSE 활성；이Evan′s람화TTC쌍염법검측심기경사체적。결과①여상응가수술조비교，결혈30 min、1、2、3、4 h조LVDP、±dp／dtmax、CF 명현강저（P＜0．01）。결혈30 min조H2S함량화CSE활성무명현변화，심기조직무명현경사조，결혈1、2、3、4 h조H2 S함량화CSE활성균명현강저（P＜0．05혹P＜0．01）。결혈1 h조가견명현경사조（P＜0．01），결혈2、3、4 h조경사체적명현증대（P＜0．01）；②여결혈4 h조비교，NaHS저、중、고제량조 LVDP、±dp／dtmax、CF 명현승고（P ＜0．05혹 P ＜0.01），H2S함량화CSE활성균승고（P＜0.05혹P＜0.01）， NaHS중、고제량조심기경사체적명현감소（P＜0.01）。결론 H2 S여CSE삼여료리체대서급성심기결혈손상。NaHS가증강급성결혈손상심기CSE활성，증가H2 S함량，사심기경사체적축소，제시H2 S가개선급성심기결혈손상。
Aims To observe the changes of endoge-nous hydrogen sulfide/cystathionine-γ-lyase (H2 S/CSE)system and to study the effects of H2 S on cardiac function,H2 S/CSE and myocardial infarct volumes in acute myocardial ischemic injury in isolated hearts of rats.Methods The myocardial ischemic injury model was established by the ligation of coronary artery.The hemodynamic parameters,such as the left ventricular developed pressure (LVDP),±dp/dtmax and coronary arterial flow(CF),were respectively recorded to evalu-ate the cardiac function.The content of H2 S and the activity of CSE in cardiac tissue were detected respec-tively at each time point after ischemia.Infarct vol-umes in isolated rat hearts were determined by dual staining with Evans-blue and TTC.Results (1 )Com-pared with those of the sham group,LVDP,±dp/dtmax and CF were significantly decreased at 30 min,1,2,3, 4 h after ischemia(P<0.01),there were no statistical-ly significant differences in the content of H2 S and the activity of CSE in cardiac tissue at 3 0 min after ische-mia.But during the periods from 1 h to 4h after ische-mia,the content of H2 S and the activity of CSE in car-diac tissue were significantly decreased,the infarct volumes were greatly increased compared with those of the sham control group (P<0.05 or P<0.01 ).(2) Compared with those of the ischemia 4h group,LVDP, ±dp/dtmax and CF were significantly increased in the NaHS low,middle and high dose groups (P<0.05 or P<0.01),the content of H2S and the activity of CSE in cardiac tissue were significantly increased in the NaHS low,middle and high dose groups(P<0.05 or P<0.01 ),and the infarct volumes were significantly decreased in NaHS middle and high dose groups(P<0.01 ).Conclusion H2S and CSE are involved in myocardial ischemic injury in isolated hearts of rats. Administration of NaHS could enhance the activity of CSE,increase the content of H2 S,and reduce infarct volumes.It could be suggested that H2 S has cardiopro-tective effects on acute myocardial ischemic injury.