CAJ | 학술논문

目的：从外周血中分离并鉴定人循环纤维细胞，初步探讨姜黄素对人循环纤维细胞的药理作用。方法密度梯度离心法分离纯化细胞，流式细胞术和免疫化学法对细胞进行鉴定；CCK-8法及流式细胞技术分别检测姜黄素对人循环纤维细胞增殖和COLⅠ表达的影响。结果在体外成功分离培养了人循环纤维细胞，细胞呈CD34、CD45和COLⅠ阳性，其中79.7％的细胞为COLⅠ和CD45双阳性，可证明为人循环纤维细胞。姜黄素对循环纤维细胞的增殖起调控作用，不同浓度姜黄素的短时间（24 h）作用可促进人循环纤维细胞的增殖；而作用较长时间（72 h）后不仅能明显抑制细胞的增殖，而且能下调COLⅠ的表达，以高浓度效果最为明显（20μmol·L－1）。结论20μmol·L－1的姜黄素作用72 h可明显抑制人循环纤维细胞的增殖和COLⅠ的表达。
목적：종외주혈중분리병감정인순배섬유세포，초보탐토강황소대인순배섬유세포적약리작용。방법밀도제도리심법분리순화세포，류식세포술화면역화학법대세포진행감정；CCK-8법급류식세포기술분별검측강황소대인순배섬유세포증식화COLⅠ표체적영향。결과재체외성공분리배양료인순배섬유세포，세포정CD34、CD45화COLⅠ양성，기중79.7％적세포위COLⅠ화CD45쌍양성，가증명위인순배섬유세포。강황소대순배섬유세포적증식기조공작용，불동농도강황소적단시간（24 h）작용가촉진인순배섬유세포적증식；이작용교장시간（72 h）후불부능명현억제세포적증식，이차능하조COLⅠ적표체，이고농도효과최위명현（20μmol·L－1）。결론20μmol·L－1적강황소작용72 h가명현억제인순배섬유세포적증식화COLⅠ적표체。
Aim To isolate and characterize the human circulating fibrocytes from human peripheral blood and explore the effects of curcumin on human circulating fi-brocytes.Methods The cells were isolated and puri-fied by density gradient centrifugation,and identified by flow cytometry and immunocytochemistry .Then , CCK-8 and flow cytometry were used to study the effect of curcumin on the proliferation as well as COL I ex-pression of human circulating fibrocytes,respectively. Results After being isolated the cells expressed CD34,CD45 and COLⅠ,among which 79.7% were both CD45 and collagen I positive,typical of human circulating fibrocytes.Curcumin could exert regulatory effects on proliferation of human circulating fibrocytes. Exposure of the cells to curcumin for as short as 24 hours promoted their growth,while prolonged treatment (72 h ) significantly inhibited cell propagation and downregulated the COLⅠ levels,best manifested at a concentration as high as 20 μmol · L-1 .Conclusion The proliferation of cells and COLⅠexpressions can be effectively inhibited by curcumin with the prolonged action period and high concentrations.