中国脑血管病杂志
中國腦血管病雜誌
중국뇌혈관병잡지
CHINESE JOURNAL OF CEREBROVASCULAR DISEASES
2014年
7期
347-353
,共7页
何芳梅%潘剑罡%赵西耀%袁华%牟翔%陈煜森
何芳梅%潘劍罡%趙西耀%袁華%牟翔%陳煜森
하방매%반검강%조서요%원화%모상%진욱삼
脑梗死%基因%凝血酶活化纤维蛋白溶解抑制物%单核苷酸多态性
腦梗死%基因%凝血酶活化纖維蛋白溶解抑製物%單覈苷痠多態性
뇌경사%기인%응혈매활화섬유단백용해억제물%단핵감산다태성
Brain infarction%Gene%Thrombin activatable fibrinolysis inhibitor%Single nucleotide polymorphism
目的：探讨中国汉族脑梗死患者凝血酶活化纤维蛋白溶解抑制物（TAFI ）基因编码区C1040T及G753A单核苷酸多态性与脑梗死的相关性。方法回顾性分析采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术，检测130例脑梗死患者和118名同期行正常体检者（对照组）的TAFI基因编码区C1040T及G753A的多态性。结果脑梗死组TAFI基因G753A多态性的GG型频率41.5％（54例），A等位基因携带者频率58.5％（76例）；而对照组分别为GG型频率44.9％（53例），A等位基因携带者频率55.1％（65例）。TAFI基因G753A多态性的GG型及A等位基因携带者两组频率差异无统计学意义（χ2＝0.288，P＝0.592）。在脑梗死组，C1040T多态性的CC型占50.0％（65例），T等位基因携带者占50.0％（65例）；而对照组分别为 CC型51.7％（61例），T等位基因携带者占48.3％（57例）。C1040T多态性的CC型及T等位基因携带者两组差异无统计学意义（χ2＝0.071，P＝0.790）。多因素Logistic回归分析显示，TAFI基因编码区G753A单核苷酸多态性（GA或AA基因型）及C1040T单核苷酸多态性（CT或TT基因型）不是脑梗死发病的独立危险因素。结论 TAFI基因编码区C1040T及G753A的多态性与脑梗死之间无显著相关性，不是脑梗死发病的独立危险因素。
目的：探討中國漢族腦梗死患者凝血酶活化纖維蛋白溶解抑製物（TAFI ）基因編碼區C1040T及G753A單覈苷痠多態性與腦梗死的相關性。方法迴顧性分析採用聚閤酶鏈反應-限製性片段長度多態性（PCR-RFLP）技術，檢測130例腦梗死患者和118名同期行正常體檢者（對照組）的TAFI基因編碼區C1040T及G753A的多態性。結果腦梗死組TAFI基因G753A多態性的GG型頻率41.5％（54例），A等位基因攜帶者頻率58.5％（76例）；而對照組分彆為GG型頻率44.9％（53例），A等位基因攜帶者頻率55.1％（65例）。TAFI基因G753A多態性的GG型及A等位基因攜帶者兩組頻率差異無統計學意義（χ2＝0.288，P＝0.592）。在腦梗死組，C1040T多態性的CC型佔50.0％（65例），T等位基因攜帶者佔50.0％（65例）；而對照組分彆為 CC型51.7％（61例），T等位基因攜帶者佔48.3％（57例）。C1040T多態性的CC型及T等位基因攜帶者兩組差異無統計學意義（χ2＝0.071，P＝0.790）。多因素Logistic迴歸分析顯示，TAFI基因編碼區G753A單覈苷痠多態性（GA或AA基因型）及C1040T單覈苷痠多態性（CT或TT基因型）不是腦梗死髮病的獨立危險因素。結論 TAFI基因編碼區C1040T及G753A的多態性與腦梗死之間無顯著相關性，不是腦梗死髮病的獨立危險因素。
목적：탐토중국한족뇌경사환자응혈매활화섬유단백용해억제물（TAFI ）기인편마구C1040T급G753A단핵감산다태성여뇌경사적상관성。방법회고성분석채용취합매련반응-한제성편단장도다태성（PCR-RFLP）기술，검측130례뇌경사환자화118명동기행정상체검자（대조조）적TAFI기인편마구C1040T급G753A적다태성。결과뇌경사조TAFI기인G753A다태성적GG형빈솔41.5％（54례），A등위기인휴대자빈솔58.5％（76례）；이대조조분별위GG형빈솔44.9％（53례），A등위기인휴대자빈솔55.1％（65례）。TAFI기인G753A다태성적GG형급A등위기인휴대자량조빈솔차이무통계학의의（χ2＝0.288，P＝0.592）。재뇌경사조，C1040T다태성적CC형점50.0％（65례），T등위기인휴대자점50.0％（65례）；이대조조분별위 CC형51.7％（61례），T등위기인휴대자점48.3％（57례）。C1040T다태성적CC형급T등위기인휴대자량조차이무통계학의의（χ2＝0.071，P＝0.790）。다인소Logistic회귀분석현시，TAFI기인편마구G753A단핵감산다태성（GA혹AA기인형）급C1040T단핵감산다태성（CT혹TT기인형）불시뇌경사발병적독립위험인소。결론 TAFI기인편마구C1040T급G753A적다태성여뇌경사지간무현저상관성，불시뇌경사발병적독립위험인소。
Objective To investigate the correlation between G753A and C1040T polymorphisms in the gene encoding region of thrombin-activatable fibrinolysis inhibitor (TAFI )and cerebral infarction in patients with cerebral infarction in Chinese Han population. Methods C1040T and G753A poly-morphisms in the TAFI gene encoding region in 130 patients with cerebral infarction and 118 healthy subjects (control group)were analyzed retrospectively and they were detected by using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP ). Results The GG genotyping of the TAFI gene G753A polymorphism in the cerebral group was 41. 5%(n=54)and the A allele carriers were 58. 5%(n=76),while those in the control group were 44. 9%(n=53)and 55. 1%(n=65)respectively. There were no significant differences in the GG genotyping of TAFI gene G753A polymorphism and the A allele carriers between the cerebral infarction group and the control group (χ2 =0. 288,P=0. 592). In the cerebral infarction group,the CC genotyping of C1040T polymorphism was 50. 0%(n=65)and T allele carriers were 50. 0%(n=65),while those in the control group were 51. 7%(n=61)and 48. 3%(n=57)respectively. There were no significant differences in the GG genotyping of C1040T polymorphism and the T allele carriers between the two groups (χ2 =0.071,P =0.790 ). Multivariate logistic regression analysis showed that G753A and C1040T single nucleotide polymorphisms (GA or AA genotype)in the TAFI gene encoding region were not the independent risk factors for cerebral infarction. Conclusion There are no significant differences in the correlation between the G753A and C1040T polymorphisms in the TAFI gene encoding region and cerebral infarction. They are not the independent risk factors for the onset of cerebral infarction.
