CAJ | 학술논문

目的：对1株蝙蝠来源病毒进行分离鉴定，进行简单的基因分析。方法从云南沧源县8个居民地附近捕获50只棕果蝠并制作组织标本，采用细胞培养分离病毒，采用 cDNA-RAPD 方法扩增序列，采用分子克隆进一步扩增序列，将序列在 NCBI 上进行 BLAST 比对，采用 DNAMAN5.0进行同源性分析，采用 MEGA 5建序列进化树。结果50份接毒细胞中，6份细胞出现细胞病变，cDNA-RAPD 扩增得到1份阳性片段，测序大小为614 bp，经比对该病毒株是细小病毒科细小病毒亚科博卡病毒属牛细小病毒种的一个毒株。结论成功分离并鉴定该毒株，确定该病毒是牛细小病毒的一个新的基因型。
목적：대1주편복래원병독진행분리감정，진행간단적기인분석。방법종운남창원현8개거민지부근포획50지종과복병제작조직표본，채용세포배양분리병독，채용 cDNA-RAPD 방법확증서렬，채용분자극륭진일보확증서렬，장서렬재 NCBI 상진행 BLAST 비대，채용 DNAMAN5.0진행동원성분석，채용 MEGA 5건서렬진화수。결과50빈접독세포중，6빈세포출현세포병변，cDNA-RAPD 확증득도1빈양성편단，측서대소위614 bp，경비대해병독주시세소병독과세소병독아과박잡병독속우세소병독충적일개독주。결론성공분리병감정해독주，학정해병독시우세소병독적일개신적기인형。
Objective To isolate and identify a virus from bats and to make simple gene analysis. Methods 50 rousettus leschenaultias were captured from the surrounding areas of 8 residences in Cangyuan County,Yunnan Province and some of their organs were collected;cell culture was adopted to isolate the virus;the sequence of the virus was first amplified by cDNA-RAPD and then further amplified by molecular cloning;the sequence was compared in the NCBI;the homology analysis of the virus was carried out by DNAMAN5. 0 and the phylogenetic analysis was executed by MEGA 5. Results Fifty cells were infected and six cells turned to be CPE and a positive gene fragment was obtained by cDNA-RAPD. The sequencing result indicated that the fragment was 614 bp long. The virus was identified to be one of the Bovine parvovirus of Bocavirus of Parvovirinae of Parvoviridae. Conclusions The isolation and identification of the virus was successful and the virus was confirmed to be a new genotype of Bovine parvovirus.