CAJ | 학술논문

目的：建立 HPLC 法测定大鼠血浆中芹菜素的浓度,研究紫花地丁中芹菜素在大鼠体内的药动学特征。方法以乙腈：0.2%磷酸水溶液(65：35)为流动相,血浆样品经乙酸乙酯萃取后,采用 HPLC 法测定芹菜素的血药浓度,检测波长340nm。采用 DAS3.0软件计算主要药动学参数。结果芹菜素在0.03~9.00μg/ mL 范围内线性关系良好(r =0.9992),方法回收率为(97.36%~101.69%),日内和日间 RSD 均<13%。主要药动学参数 C max 为(220.59±58.65)μg/ mL,t 1/2为(6.785±2.833)h,AUC (0-18)为(11.42±3.587)μg/(mL·h),AUC (0-∞)为(22.84±6.572)μg/(mL·h)。结论芹菜素在大鼠体内过程符合一室模型,其药动学研究结果为紫花地丁进一步体内研究奠定了良好基础。
목적：건립 HPLC 법측정대서혈장중근채소적농도,연구자화지정중근채소재대서체내적약동학특정。방법이을정：0.2%린산수용액(65：35)위류동상,혈장양품경을산을지췌취후,채용 HPLC 법측정근채소적혈약농도,검측파장340nm。채용 DAS3.0연건계산주요약동학삼수。결과근채소재0.03~9.00μg/ mL 범위내선성관계량호(r =0.9992),방법회수솔위(97.36%~101.69%),일내화일간 RSD 균<13%。주요약동학삼수 C max 위(220.59±58.65)μg/ mL,t 1/2위(6.785±2.833)h,AUC (0-18)위(11.42±3.587)μg/(mL·h),AUC (0-∞)위(22.84±6.572)μg/(mL·h)。결론근채소재대서체내과정부합일실모형,기약동학연구결과위자화지정진일보체내연구전정료량호기출。
Objective To establish a HPLC method to detect the concentration of Pelargidenon in rats. And to study the pharmacokinetics of Pelargidenon in Tokyo violet herb. Methods A mixture of acetonitrile and 0. 2% phosphoric acid (65 : 35) was used as the mobile phase. After extracted by acetic ether, plasma samples were determined by HPLC at 340nm. And the phar-macokinetic parameters were calculated by DAS 3. 0 software. Results The range of liner was 0. 03 ~ 9. 00μg/ mL (r = 0. 9992). The method recovery was 97. 36% ~ 101. 69% . Both the within-day and between-day RSD were less than 13% . The main pharma-cokinetic parameters were as follows:C max (220. 59 ± 58. 65) μg/ mL, t 1 / 2 (6. 785 ± 2. 833) h, AUC (0-18) (11. 42 ± 3. 587)μg/ (mL·h), AUC (0- ∞ ) (22. 84 ± 6. 572) μg/ (mL·h). Conclusion The plasma concentration-time curve of Pelargidenon was fitted to one-compartment open pharmacokinetic model. This pharmacokinetic study of Pelargidenon provided a good foundation for the further in vivo study of Tokyo violet herb.