粮食与油脂
糧食與油脂
양식여유지
CEREALS & OILS
2014年
7期
28-32
,共5页
陈云%李文治%罗其昌%高群玉
陳雲%李文治%囉其昌%高群玉
진운%리문치%라기창%고군옥
菊花%多糖%水浸提法%超声波辅助法%酶法
菊花%多糖%水浸提法%超聲波輔助法%酶法
국화%다당%수침제법%초성파보조법%매법
chrysanthemum%polysaccharides%water extraction%ultrasonic-assisted extraction%enzyme extraction
以菊花（产自杭州的杭白菊）为材料，比较水浸提法、超声波辅助提取法和酶辅助提取法对菊花多糖得率的影响，得出最优提取方法和提取条件。考察了水浸提法中提取时间、料液比和提取温度对多糖得率的影响，得出最优水提工艺为提取时间2 h、料液比1∶25（W/V）、提取温度90℃；考察了超声波辅助提取法中提取时间、料液比、提取温度和功率对多糖得率的影响，得到最优条件为提取时间35 min、料液比1∶25、提取温度70℃、提取功率60 W；考察了酶提取法中纤维素酶水解提取菊花多糖提取时间、料液比和酶用量对多糖得率的影响，得出的最优条件为提取时间2 h、物料比1∶40、加酶量占样品重的2%。结果表明，在所设定的实验条件下菊花多糖的得率，以酶提取法最高，超声波辅助提取法次之，水浸提法最低。
以菊花（產自杭州的杭白菊）為材料，比較水浸提法、超聲波輔助提取法和酶輔助提取法對菊花多糖得率的影響，得齣最優提取方法和提取條件。攷察瞭水浸提法中提取時間、料液比和提取溫度對多糖得率的影響，得齣最優水提工藝為提取時間2 h、料液比1∶25（W/V）、提取溫度90℃；攷察瞭超聲波輔助提取法中提取時間、料液比、提取溫度和功率對多糖得率的影響，得到最優條件為提取時間35 min、料液比1∶25、提取溫度70℃、提取功率60 W；攷察瞭酶提取法中纖維素酶水解提取菊花多糖提取時間、料液比和酶用量對多糖得率的影響，得齣的最優條件為提取時間2 h、物料比1∶40、加酶量佔樣品重的2%。結果錶明，在所設定的實驗條件下菊花多糖的得率，以酶提取法最高，超聲波輔助提取法次之，水浸提法最低。
이국화（산자항주적항백국）위재료，비교수침제법、초성파보조제취법화매보조제취법대국화다당득솔적영향，득출최우제취방법화제취조건。고찰료수침제법중제취시간、료액비화제취온도대다당득솔적영향，득출최우수제공예위제취시간2 h、료액비1∶25（W/V）、제취온도90℃；고찰료초성파보조제취법중제취시간、료액비、제취온도화공솔대다당득솔적영향，득도최우조건위제취시간35 min、료액비1∶25、제취온도70℃、제취공솔60 W；고찰료매제취법중섬유소매수해제취국화다당제취시간、료액비화매용량대다당득솔적영향，득출적최우조건위제취시간2 h、물료비1∶40、가매량점양품중적2%。결과표명，재소설정적실험조건하국화다당적득솔，이매제취법최고，초성파보조제취법차지，수침제법최저。
Three different procedures of hot water,ultrasonic-assisted and enzyme for the extraction of polysaccharide from Chrysanthemum were studied. The three procedures were compared and then the best procedure and the optimum extraction conditions were obtained. The hot water extraction conditions of chrysanthemum polysaccharides were investigated. Three parameters affecting the extraction efficiency of polysaccharides were extraction times,ratio of water to raw material and extraction temperature. The optimum extraction conditions were found to be extraction time 2 h,ratio of water to raw material 25(W/V),extraction temperature 90℃. The parameters of ultrasonic-assisted extraction on extraction efficiency of polysaccharides were extraction time,ratio of water to raw material,extraction temperature and ultrasonic power and the optimum extraction conditions of ultrasonic-assisted extraction were extraction time 35 min,ratio of water to raw material 25,extraction temperature 70℃and ultrasonic power 60 W. The cellulase extraction conditions of chrysanthemum polysaccharides were investigated too. Three parameters affecting the extraction efficiency of polysaccharides were extraction time,ratio of water to raw material and the ratio of celluase to raw material. The optimum conditions of celluase extraction were extraction time 2 h,ratio of water to raw material 40 and the ratio of celluase to raw material 0.02. As the result,the best procedure for the extraction of polysaccharide from chrysanthemum was the enzyme extraction.
