CAJ | 학술논문

以两种典型的陆生植物小麦和玉米为供试材料，采用盆栽实验生物富积方式，对比研究了薄膜扩散梯度（DGT）技术与5种传统的化学提取方法评价铅（Pb）复合存在下土壤镉（Cd）的生物有效性。结果表明，Cd污染土壤中Pb的存在促进两种植物对Cd的吸收，并随Pb浓度的增加其促进作用更趋明显；DGT技术测定的土壤有效态Cd含量随土壤Pb浓度的增加而显著增加，土壤溶液中Cd的浓度和4种单一提取剂（醋酸、氯化钙、醋酸钠和乙二胺四乙酸）提取的土壤有效态Cd含量均与DGT技术表现出类似的变化趋势，从动力学和静态平衡两种角度揭示了土壤Pb的存在增强Cd的生物有效性；Pearson回归分析显示，6种方法表征的土壤有效态Cd含量均与两种植物体内Cd的含量呈显著正相关，但DGT、土壤溶液和CaCl2的相关系数明显高于其他3种化学提取方法。结果显示，DGT技术与传统方法（土壤溶液法和CaCl2提取法）均可用于预测土壤Cd的生物有效性。
이량충전형적륙생식물소맥화옥미위공시재료，채용분재실험생물부적방식，대비연구료박막확산제도（DGT）기술여5충전통적화학제취방법평개연（Pb）복합존재하토양력（Cd）적생물유효성。결과표명，Cd오염토양중Pb적존재촉진량충식물대Cd적흡수，병수Pb농도적증가기촉진작용경추명현；DGT기술측정적토양유효태Cd함량수토양Pb농도적증가이현저증가，토양용액중Cd적농도화4충단일제취제（작산、록화개、작산납화을이알사을산）제취적토양유효태Cd함량균여DGT기술표현출유사적변화추세，종동역학화정태평형량충각도게시료토양Pb적존재증강Cd적생물유효성；Pearson회귀분석현시，6충방법표정적토양유효태Cd함량균여량충식물체내Cd적함량정현저정상관，단DGT、토양용액화CaCl2적상관계수명현고우기타3충화학제취방법。결과현시，DGT기술여전통방법（토양용액법화CaCl2제취법）균가용우예측토양Cd적생물유효성。
Diffusive gradients in thin films(DGT)technique has recently been recommended as a tool for predicating heavy metal availabili-ty in soils. However, there are still debates on its applicability. In this study, pot experiments were conducted to measure Cd bioavailability under combined pollution of Cd and Pb in soils. The Cd bioavailability was then evaluated by DGT and five chemical extraction methods in-cluding soil solution, HAc, EDTA, CaCl2 and NaAc). Presence of Pb enhanced Cd uptake by shoots and roots of wheat and maize in Pb-and Cd-contaminated soils. Such enhancement of plant Cd uptake increased with increasing Pb concentrations. Correspondingly, bioavail-able Cd concentrations measured with DGT(CDGT), soil solution Cd(CSOL), and HAc , EDTA , NaAc and CaCl2- extractable Cd all showed similar trends as plant Cd uptake . Pearson correlation analysis showed that significant and positive correlations existed between soil bioavailable Cd estimated by each method and Cd concentrations in two plants, but the correlation coefficients of DGT, CSOL and CaCl2 were greater than those of HAc, EDTA and NaAc. These results suggest that DGT and traditional extraction methods(soil solution and CaCl2) could be used to predict soil Cd bioavailability to plants.