CAJ | 학술논문

通过模拟土壤溶液进行水培试验，研究了锌（Zn）对大麦（Hordeum V ulgare）镉（Cd）、铜（Cu）毒性的影响，以及Zn-Cd和Zn-Cu的联合毒性效应。结果表明：Zn、Cd和Cu单独作用于大麦时，EC50分别为78.01、2.98、0.64μmol·L-1，Zn的毒性远远小于Cd和Cu。在Zn-Cd混合体系中，当Zn浓度低于5μmol·L-1时，随着溶液中Zn浓度的增加，EC50（Cd）呈显著的线性增加，表明低浓度Zn对大麦Cd毒性具有抑制作用；利用毒性单位（TU）模型计算了Zn-Cd混合体系的TUmix值，结果显示TUmix≥1 TU，且TUmix随Zn浓度的升高而增加，表明Zn、Cd联合作用于大麦时主要表现为拮抗作用，且这种作用和混合体系中Zn的比例有关。在Zn-Cu混合体系中，随着Zn浓度的增加，大麦相对根长（RE）明显减小，EC50（Cu）逐渐降低，表明Zn增强了大麦Cu的毒性；Zn-Cu的TUmix均小于1 TU，Zn、Cu联合作用于大麦时表现为协同毒性作用。
통과모의토양용액진행수배시험，연구료자（Zn）대대맥（Hordeum V ulgare）력（Cd）、동（Cu）독성적영향，이급Zn-Cd화Zn-Cu적연합독성효응。결과표명：Zn、Cd화Cu단독작용우대맥시，EC50분별위78.01、2.98、0.64μmol·L-1，Zn적독성원원소우Cd화Cu。재Zn-Cd혼합체계중，당Zn농도저우5μmol·L-1시，수착용액중Zn농도적증가，EC50（Cd）정현저적선성증가，표명저농도Zn대대맥Cd독성구유억제작용；이용독성단위（TU）모형계산료Zn-Cd혼합체계적TUmix치，결과현시TUmix≥1 TU，차TUmix수Zn농도적승고이증가，표명Zn、Cd연합작용우대맥시주요표현위길항작용，차저충작용화혼합체계중Zn적비례유관。재Zn-Cu혼합체계중，수착Zn농도적증가，대맥상대근장（RE）명현감소，EC50（Cu）축점강저，표명Zn증강료대맥Cu적독성；Zn-Cu적TUmix균소우1 TU，Zn、Cu연합작용우대맥시표현위협동독성작용。
Metal toxicity to plants is different in single system from in multiple metal system. Here toxicities of individual Zn, Cd and Cu and Zn-Cd and Zn-Cu mixtures to barley(Hordeum Vulgare)were tested in nutrient solutions. Toxic unit(TU)model was used to evaluate the combined toxicity of mixture metals. EC50s(metal concentration at which root elongation is inhibited by 50%)were 78.01 μmol·L- 1, 2.98μmol·L-1 and 0.64μmol·L-1 for Zn, Cd and Cu, respectively, indicating that Cu toxicity was much higher than Cd or Zn in single-met-al system. In binary metal systems, Cd toxicity to barley was inhibited by Zn at 5μmol·L-1, and the values of EC50(Cd)increased signifi-cantly. TUmix values of Zn-Cd were greater than 1 TU and increased with Zn concentrations, suggesting an antagonistic effect between Zn and Cd at low Zn concentrations. Such antagonism of Zn-Cd was correlated with Zn proportion in the mixture system. At higher concentra-tions(﹥5μmol·L-1), Zn exerted high toxicity to barley and increased the joint toxicity of Zn-Cd. Additions of Zn showed greater Cu toxicity to barley than Cu alone. EC50 (Cu)values were higher in Zn-Cu mixture than in single-metal system and decreased dramatically with in-creasing Zn concentrations, showing synergistic toxicity of Zn-Cu. TUmix values of Zn-Cu mixture were less than 1 TU. These results suggest that Cd and Cu may have different toxicity mechanisms, and that more attention should be paid to combined heavy metal pollution.