CAJ | 학술논문

目的：采用RNAi技术沉默LIMK2基因来抑制细胞骨架改建，观察其对流体剪切力下成骨细胞COX-2基因力学敏感性的影响。方法；RNAi技术沉默原代小鼠成骨细胞LIMK2基因来抑制细胞骨架改建，分别用实时荧光定量巢式PCR和免疫荧光的方法检测流体剪切力下COX-2基因mRNA和蛋白的表达水平。结果：RNAi抑制细胞骨架改建可以促进流体剪切力诱导的成骨细胞COX-2 mRNA和蛋白的表达(P<0.05)。结论：采用RNAi技术抑制细胞骨架改建，可以提高流体剪切力作用下成骨细胞COX-2基因的力学敏感性。
목적：채용RNAi기술침묵LIMK2기인래억제세포골가개건，관찰기대류체전절력하성골세포COX-2기인역학민감성적영향。방법；RNAi기술침묵원대소서성골세포LIMK2기인래억제세포골가개건，분별용실시형광정량소식PCR화면역형광적방법검측류체전절력하COX-2기인mRNA화단백적표체수평。결과：RNAi억제세포골가개건가이촉진류체전절력유도적성골세포COX-2 mRNA화단백적표체(P<0.05)。결론：채용RNAi기술억제세포골가개건，가이제고류체전절력작용하성골세포COX-2기인적역학민감성。
Objective: To study the effect of cytoskelet on reorganization in hibition with RNA interference on the mechanosensitivity of COX -2 gene in osteoblasts induced by fluid shear stress.Methods: RNA interference against LIMK2 gene was used to inhibit the cytoskeleton reorganization of BALB/cmouse prim ary osteoblasts.Osteoblasts were loaded by fluid shear stress at12 dyne/cm 2 or unloaded.The nested real-time PCR and immun of luorescence were performed to detect the expression levels of COX-2m RNA and protein, respectively. Result: Cytoskeleton reorganization inhibition with RNA interference against LIMK2 gene increased the expression of COX -2 gene induced by fluid shear stress.Conclusion: Cytoskeleton reorganization inhibition with RNA interference against LIMK2 gene could enhance the mechanosensitivity of COX -2 gene induced by fluid shearstress.