CAJ | 학술논문

为了探索重金属镉对烟草的危害机理，采用水培试验，调查了不同镉浓度处理在烟草幼苗各部位不同提取态的镉含量以及烟草幼苗干物质积累量、根系活力、叶绿素含量、硝酸还原酶活力和丙二醛含量等生理指标的变化。结果表明，烟草根系镉（Cd）以去离子水提取态为主，其次为醋酸提取态，叶片中Cd主要以醋酸结合态存在。随着镉浓度的提高，Cd存在由活性弱的化学形态向活性强的形态转化的趋势，干物质量的积累先升高后降低；不同Cd浓度处理时，烟草幼苗Cd含量表现为：茎>根>叶，随着Cd处理浓度的提高，烟草幼苗中的Cd更多的富集在烟苗的根部和茎部。根系活力和叶绿素含量逐渐降低；硝酸还原酶活性呈先升高后降低，丙二醛含量则反之。因此，与对照相比，低浓度Cd处理在一定程度上刺激烟草幼苗的某些生理活性，使其呈现更高的生物活性，但高浓度Cd处理则极显著抑制烟草幼苗的生长，使其各项生理活动降低，最终影响其正常的生长。
위료탐색중금속력대연초적위해궤리，채용수배시험，조사료불동력농도처리재연초유묘각부위불동제취태적력함량이급연초유묘간물질적루량、근계활력、협록소함량、초산환원매활력화병이철함량등생리지표적변화。결과표명，연초근계력（Cd）이거리자수제취태위주，기차위작산제취태，협편중Cd주요이작산결합태존재。수착력농도적제고，Cd존재유활성약적화학형태향활성강적형태전화적추세，간물질량적적루선승고후강저；불동Cd농도처리시，연초유묘Cd함량표현위：경>근>협，수착Cd처리농도적제고，연초유묘중적Cd경다적부집재연묘적근부화경부。근계활력화협록소함량축점강저；초산환원매활성정선승고후강저，병이철함량칙반지。인차，여대조상비，저농도Cd처리재일정정도상자격연초유묘적모사생리활성，사기정현경고적생물활성，단고농도Cd처리칙겁현저억제연초유묘적생장，사기각항생리활동강저，최종영향기정상적생장。
In order to explore the damage mechanism of cadmium (Cd) on tobacco, effects of cadmium with different concentrations on the growth and the physiological and biochemical activities of tobacco were studied by solution culture. The results showed that the growth of tobacco plant was restrained under the cadmium stress. Acetic acid-extractable Cd was the main chemical form of Cd in root system. In leaves, Cd mainly existed in the forms of binding to Acetic Acid and deionized water. With the increase of Cd concentration, Cd tended to transform from low activity to high activity. The Cd content in different parts of tobacco seedlings under different Cd treatment was: stem > root > leaf. The chlorophyll content, nitrate reductase activity, root activity and MDA content changed under different cadmium treatment to tobacco seedlings. With the increase of cadmium concentration, the dry matter accumulation increased firstly and then decreased, chlorophyll content and root activity showed a gradual decreasing trend, nitrate reductase activity increased firstly and then decreased, and MDA content decreased first and then increased. This study suggest that low concentrations of Cd can stimulate tobacco biological activity, but high concentrations of Cd can significantly inhibite the growth of tobacco seedlings, reduce the physiological activity, and ultimately affect their normal growth.