中国烟草科学
中國煙草科學
중국연초과학
CHINESE TOBACCO SCIENCE
2014年
2期
26-31
,共6页
陈壮壮%谢虹%钟宇%张鹏远%龙春瑞%陈林%王建光%陈穗云%李秀军
陳壯壯%謝虹%鐘宇%張鵬遠%龍春瑞%陳林%王建光%陳穗雲%李秀軍
진장장%사홍%종우%장붕원%룡춘서%진림%왕건광%진수운%리수군
青霉菌灭活菌丝体%烤烟%红花大金元%cDNA文库%SSH
青黴菌滅活菌絲體%烤煙%紅花大金元%cDNA文庫%SSH
청매균멸활균사체%고연%홍화대금원%cDNA문고%SSH
Dry Mycelium of Penicillium Chrysogenum%Nicotiana tabacum%Honghuadajinyuan%cDNA library%SSH
为了探究青霉菌灭活菌丝体(DMP)在烟草抗性中的作用机制,以DMP水溶液处理的烟草红花大金元叶片为材料,利用抑制消减杂交(SSH)技术构建了cDNA文库,并对文库进行了分析。SSH获得356个克隆,挑选102个克隆,测序成功77个。Blastn比对出47个不同功能的基因。Blastx比对出26个不同功能的蛋白;7种未知功能的预测蛋白;2个克隆没有比对出相关蛋白。所有克隆抗病防御、能量代谢和细胞保护相关基因较多,分别占总数的40.3%、23.4%和14.9%。其余有关细胞结构、蛋白合成、转录及信号传导的基因相对较少,分别占到8.5%、4.3%、4.3%和4.3%。
為瞭探究青黴菌滅活菌絲體(DMP)在煙草抗性中的作用機製,以DMP水溶液處理的煙草紅花大金元葉片為材料,利用抑製消減雜交(SSH)技術構建瞭cDNA文庫,併對文庫進行瞭分析。SSH穫得356箇剋隆,挑選102箇剋隆,測序成功77箇。Blastn比對齣47箇不同功能的基因。Blastx比對齣26箇不同功能的蛋白;7種未知功能的預測蛋白;2箇剋隆沒有比對齣相關蛋白。所有剋隆抗病防禦、能量代謝和細胞保護相關基因較多,分彆佔總數的40.3%、23.4%和14.9%。其餘有關細胞結構、蛋白閤成、轉錄及信號傳導的基因相對較少,分彆佔到8.5%、4.3%、4.3%和4.3%。
위료탐구청매균멸활균사체(DMP)재연초항성중적작용궤제,이DMP수용액처리적연초홍화대금원협편위재료,이용억제소감잡교(SSH)기술구건료cDNA문고,병대문고진행료분석。SSH획득356개극륭,도선102개극륭,측서성공77개。Blastn비대출47개불동공능적기인。Blastx비대출26개불동공능적단백;7충미지공능적예측단백;2개극륭몰유비대출상관단백。소유극륭항병방어、능량대사화세포보호상관기인교다,분별점총수적40.3%、23.4%화14.9%。기여유관세포결구、단백합성、전록급신호전도적기인상대교소,분별점도8.5%、4.3%、4.3%화4.3%。
Dry mycelium of Penicillium chrysogenum (DMP) could protect plant from disease. But its mechanism of induction and signaling pathways was unclear. Analyzing the genes expression in the plant treated by DMP was a good way to find out how inducing agent works. SSH technology was used to establish a cDNA library of tobacco induced by Dry mycelium of Penicillium chrysogenum. In the library 356 positive clones were obtained, of which 102 clones were selected for sequencing and 77 were sequenced successful. The result of Blastn showed that 47 genes’ functions were obtained in the library. The result of Blastx indicated that 26 genes had homologous sequences and 7 genes showed high similarities with putative proteins; 2 genes did not get any homologous sequence or protein. Defense-related genes (40.3%) were most in the library; The energy metabolism and the cytoprotective genes were in the next place (23.4%and 14.9%respectively). The genes related to eucaryotic cell structure, protein synthesis, transduction and signal transduction were fewer (8.5%, 4.3%, 4.3%and 4.3%respectively). DMP can induce numerous genes expression in tobacco. Masses of genes are related to plant growth and stress resistance.