CAJ | 학술논문

目的：观察靶向干扰叉头框E1（forkhead box E1，FOXE1）基因对人甲状腺乳头状癌（papil-lary thyroid carcinoma，PTC）TPC-1细胞上皮间质转化（epithelial to mesenchymal transition，EMT）的影响，探讨其促进肿瘤侵袭迁移的作用机制。方法应用慢病毒介导的RNA干扰技术沉默FOXE1基因表达，用定量PCR及Western blot方法检测干扰FOXE1后TPC-1细胞EMT标志物E-cadherin、N-cadherin和Vim-entin的表达改变。 Transwell侵袭实验和划痕实验检测干扰FOXE1基因后PTC细胞TPC-1的运动、侵袭能力变化。结果干扰FOXE1基因表达后，细胞形态出现EMT变化，上皮表型标志物E-cadherin表达明显降低，而间质表型Vimentin表达显著上升，差异有统计学意义（P＜0.01），实验组细胞的Vimentin mR-NA表达水平为对照组的2.24倍；同时，PTC细胞TPC-1的运动及侵袭能力明显增强，穿过小室的细胞数为对照组的2.11倍。结论特异性沉默FOXE1基因，可能通过EMT增强PTC细胞的侵袭潜能。
목적：관찰파향간우차두광E1（forkhead box E1，FOXE1）기인대인갑상선유두상암（papil-lary thyroid carcinoma，PTC）TPC-1세포상피간질전화（epithelial to mesenchymal transition，EMT）적영향，탐토기촉진종류침습천이적작용궤제。방법응용만병독개도적RNA간우기술침묵FOXE1기인표체，용정량PCR급Western blot방법검측간우FOXE1후TPC-1세포EMT표지물E-cadherin、N-cadherin화Vim-entin적표체개변。 Transwell침습실험화화흔실험검측간우FOXE1기인후PTC세포TPC-1적운동、침습능력변화。결과간우FOXE1기인표체후，세포형태출현EMT변화，상피표형표지물E-cadherin표체명현강저，이간질표형Vimentin표체현저상승，차이유통계학의의（P＜0.01），실험조세포적Vimentin mR-NA표체수평위대조조적2.24배；동시，PTC세포TPC-1적운동급침습능력명현증강，천과소실적세포수위대조조적2.11배。결론특이성침묵FOXE1기인，가능통과EMT증강PTC세포적침습잠능。
Objective To investigate the effects of targeted forkhead box E1( FOXE1) gene on epitheli-al-mesenchymal transition(EMT) of papillary thyroid cancer (PTC) cell line TPC-1, and to study its role in in-vasion and migration of TPC-1 cells.Methods The lentiviral expression vector for RNA interference of FOXE 1 gene was constructed to silence the expression of FOXE 1.Real-time polymerase chain reaction ( RT-PCR) and Western blot were used to detect the expression of such EMT markers as E-cadherin , N-cadherin and Vimentin . Transwell assay and Scratch assay were used to analyze TPC-1 cells migration and invasion .Results After RNA interference of FOXE1, the morphology of TPC-1 cells indicated a transformation of EMT .The expression of epi-thelial phenotype marker E-cadherin decreased remarkably while mesenchymal marker Vimentin was significantly up-regulated(P<0.01).Compared with the control group , the expression of Vimentin mRNA in the experimen-tal group was 2.24 times higher .The migration and invasion ability of TPC-1 cells increased significantly , and the number of cells in transwell was 2.11 times of the control's.Conclusion The silence of FOXE1 gene probably increases the invasion potential of human PTC cells through EMT .