CAJ | 학술논문

【目的】探讨规模猪场不同免疫次数对口蹄疫病毒（FMDV）抗体产生的影响，为指导口蹄疫（FMD）免疫提供理论依据。【方法】以存栏母猪100头为分界，从广西16家不同规模养猪场采集经FMD疫苗一免、二免和三免后1个月的血清，共720份，然后使用ELISA试剂盒对血清样品的FMDV抗体水平进行检测。【结果】两种规模猪场猪群一免、二免和三免后1个月产生的FMDV抗体总合格率分别为52.08%、72.08%和86.67%，对应平均抗体离散度分别为53.83%、75.75%和106.46%，S/N值分别为0.527、0.387和0.294。其中，一免和三免的FMDV抗体合格率、S/N值差异极显著（P<0.01），FMDV抗体离散度差异显著（P<0.05）。从猪场规模分析发现，100头母猪以上猪场的FMDV抗体平均阳性率为75.83%，抗体离散度为87.71%，平均S/N值为0.350；而100头母猪以下猪场的FMDV抗体平均阳性率为64.72%，抗体离散度为67.47%，平均S/N值为0.455。不同规模猪场猪群的FMDV抗体合格率及S/N值差异极显著（P<0.01），但离散度差异不显著（P>0.05）。【结论】FMD免疫一次远达不到国家要求的免疫效果，经过三免后FMD免疫效果最佳。实际生产中，规模化猪场应加强对猪群FMDV抗体的监测，并根据抗体消长规律有针对性地进行免疫，提高猪群抗体水平的均匀度，有效防控FMD流行。
【목적】탐토규모저장불동면역차수대구제역병독（FMDV）항체산생적영향，위지도구제역（FMD）면역제공이론의거。【방법】이존란모저100두위분계，종엄서16가불동규모양저장채집경FMD역묘일면、이면화삼면후1개월적혈청，공720빈，연후사용ELISA시제합대혈청양품적FMDV항체수평진행검측。【결과】량충규모저장저군일면、이면화삼면후1개월산생적FMDV항체총합격솔분별위52.08%、72.08%화86.67%，대응평균항체리산도분별위53.83%、75.75%화106.46%，S/N치분별위0.527、0.387화0.294。기중，일면화삼면적FMDV항체합격솔、S/N치차이겁현저（P<0.01），FMDV항체리산도차이현저（P<0.05）。종저장규모분석발현，100두모저이상저장적FMDV항체평균양성솔위75.83%，항체리산도위87.71%，평균S/N치위0.350；이100두모저이하저장적FMDV항체평균양성솔위64.72%，항체리산도위67.47%，평균S/N치위0.455。불동규모저장저군적FMDV항체합격솔급S/N치차이겁현저（P<0.01），단리산도차이불현저（P>0.05）。【결론】FMD면역일차원체불도국가요구적면역효과，경과삼면후FMD면역효과최가。실제생산중，규모화저장응가강대저군FMDV항체적감측，병근거항체소장규률유침대성지진행면역，제고저군항체수평적균균도，유효방공FMD류행。
[Objective]Effects of different immunization times on FMDV antibodies from pigs in intensive pig farms were studied to provide references for FMDV immunization. [Method]Taking 100 sows as a boundary, 720 pig serum samples were collected from 16 farms. These serum samples had been collected after one month of prime, first boost and second boost for FMD vaccination, respectively. The antibody levels of these serum samples were measured by blocking-ELISA technique.[Result]The ratio of antibody positive of prime, first boost and second boost for FMD was 52.08%,72.08%and 86.67%, and dispersion was 53.83%,75.75%and 106.46%. S/N level was 0.527, 0.387 and 0.294, respectively. The ratio of antibody positive of prime and second boost and S/N level were extremely significantly different (P<0.01). The ratio of antibody positive of prime and second boost and dispersion were significantly different (P<0.05). Average antibody positive ratio, dispersion and S/N of those farms with more than 100 sows was 75.83%, 87.71%and 0.350, while for farms with less than 100 sows, average anti-body positive ratio, dispersion and S/N was 64.72%, 67.47%and 0.455, respectively. There was extremely significant differ-ence (P<0.01) between FMD antibody levels and S/N value from different scales of farms, but there was no significant differ-ence between FMD antibody levels and dispersion (P>0.05). [Conclusion]The prime immunization could not meet national standards. The second boost immunization showed the best antibody levels. In practical operation, intensive pig farms should enhance the monitoring of FMDV antibodies, optimize the vaccination procedures based on the results of antibody monitoring and improve antibody uniformity in different herds of pigs to effectively control and prevent FMD prevalence.