南方农业学报
南方農業學報
남방농업학보
GUANGXI AGRICULTURAL SCIENCES
2014年
3期
475-478
,共4页
黄海婷%钟霞军%顾敬文%王柳萍
黃海婷%鐘霞軍%顧敬文%王柳萍
황해정%종하군%고경문%왕류평
石韦%绿原酸%高效液相色谱法(HPLC)%广西
石韋%綠原痠%高效液相色譜法(HPLC)%廣西
석위%록원산%고효액상색보법(HPLC)%엄서
Pyrrosia%chlorogenic acid%HPLC%Guangxi
【目的】采用高效液相色谱法（HPLC）测定分析广西不同产地石韦药材绿原酸含量的差异，为广西石韦药材资源的合理开发与利用提供参考。【方法】采用HPLC测定广西不同产地石韦药材绿原酸的含量，具体的色谱条件：色谱柱为C18，流动相为乙腈-0.05%磷酸（11.5∶88.5），检测波长326 nm，流速1.0 mL/min，柱温30℃。【结果】绿原酸在0.0368~0.8096μg范围内线性关系良好，回归方程为y=2607.6x+57.476（R2=0.9999），平均回收率为100.08%，RSD=0.72%。广西不同产地石韦药材绿原酸含量存在较大差异，其中，以采自金秀县罗香乡的石韦绿原酸含量最高，为3.6967 mg/g；其次是采自资源县宝鼎瀑布景区和资源县龙洞峡景区的石韦，其绿原酸含量分别为3.4678和3.1676 mg/g；绿原酸含量最低的是靖西县柔软石韦（1.1326 mg/g）。【结论】以HPLC测定石韦绿原酸具有操作简便、结果准确、重现性好等优点，是鉴别石韦品种及评价石韦药材质量的重要检测方法。广西不同产地石韦药材绿原酸含量存在较大差异，可能与产地、品种及采收时间等因素有关。
【目的】採用高效液相色譜法（HPLC）測定分析廣西不同產地石韋藥材綠原痠含量的差異，為廣西石韋藥材資源的閤理開髮與利用提供參攷。【方法】採用HPLC測定廣西不同產地石韋藥材綠原痠的含量，具體的色譜條件：色譜柱為C18，流動相為乙腈-0.05%燐痠（11.5∶88.5），檢測波長326 nm，流速1.0 mL/min，柱溫30℃。【結果】綠原痠在0.0368~0.8096μg範圍內線性關繫良好，迴歸方程為y=2607.6x+57.476（R2=0.9999），平均迴收率為100.08%，RSD=0.72%。廣西不同產地石韋藥材綠原痠含量存在較大差異，其中，以採自金秀縣囉香鄉的石韋綠原痠含量最高，為3.6967 mg/g；其次是採自資源縣寶鼎瀑佈景區和資源縣龍洞峽景區的石韋，其綠原痠含量分彆為3.4678和3.1676 mg/g；綠原痠含量最低的是靖西縣柔軟石韋（1.1326 mg/g）。【結論】以HPLC測定石韋綠原痠具有操作簡便、結果準確、重現性好等優點，是鑒彆石韋品種及評價石韋藥材質量的重要檢測方法。廣西不同產地石韋藥材綠原痠含量存在較大差異，可能與產地、品種及採收時間等因素有關。
【목적】채용고효액상색보법（HPLC）측정분석엄서불동산지석위약재록원산함량적차이，위엄서석위약재자원적합리개발여이용제공삼고。【방법】채용HPLC측정엄서불동산지석위약재록원산적함량，구체적색보조건：색보주위C18，류동상위을정-0.05%린산（11.5∶88.5），검측파장326 nm，류속1.0 mL/min，주온30℃。【결과】록원산재0.0368~0.8096μg범위내선성관계량호，회귀방정위y=2607.6x+57.476（R2=0.9999），평균회수솔위100.08%，RSD=0.72%。엄서불동산지석위약재록원산함량존재교대차이，기중，이채자금수현라향향적석위록원산함량최고，위3.6967 mg/g；기차시채자자원현보정폭포경구화자원현룡동협경구적석위，기록원산함량분별위3.4678화3.1676 mg/g；록원산함량최저적시정서현유연석위（1.1326 mg/g）。【결론】이HPLC측정석위록원산구유조작간편、결과준학、중현성호등우점，시감별석위품충급평개석위약재질량적중요검측방법。엄서불동산지석위약재록원산함량존재교대차이，가능여산지、품충급채수시간등인소유관。
[Objective]Using HPLC method, the content of chlorogenic acid in Pyrrosia produced from different areas of Guangxi was determined to provide references for appropriate utilization and development of Pyrrosia medicinal materials in Guangxi. [Method]The content of chlorogenic acid in Pyrrosia was determined by HPLC. Chromatographic conditions were as follows. Chromatographic column was C18. Mobile phase was acetonitrile-0.05% phosphoric acid (11.5∶88.5). Detection wavelength was 326 nm. Flow rate was 1.0 mL/min. Column temperature was 30℃. [Result]There was good linear relation-ship of chlorogenic acid in the range of 0.0368-0.8096μg. The regression equation was y=2607.6x+57.476 (R2=0.9999). Av-erage recovery rate was 100 . 08% ( RSD=0 . 72%) . Vast differences of chlorogenic acid were observed in Pyrrosia from different areas of Guangxi. The highest content of chlorogenic acid was found in Pyrrosia from Luoxiang Village of Jinxiu County (3.6967 mg/g) followed by Pyrrosia from Baoding Waterfall and Dragon Cave scenic region of Ziyuan County with the chlorogenic acid content of 3.4678 and 3.1676 mg/g, respectively. The lowest content of chlorogenic acid was shown in Pyrrosia porosa from Jingxi County (1.1326 mg/g).[Conclusion]The HPLC method of measuring chlorogenic acid was simple, accurate and reproductive, thus it’s an important detection method for identifying and evaluating Pyrrosia medicinal materials. The con-tent of chlorogenic acid varied in Pyrrosia from different areas of Guangxi, which may be caused by origin, cultivar or harvest time.
