CAJ | 학술논문

【目的】从药用植物鸦胆子中分离对植物病原真菌具有拮抗活性的内生细菌，为新型生物杀菌剂的开发提供依据。【方法】采用组织匀浆法对鸦胆子内生细菌进行分离，通过离体和活体组织抑菌试验筛选活性菌株；根据形态特征、生理生化测定、16S rDNA序列分析和Biolog系统对筛选获得的高活性菌株进行鉴定。【结果】从鸦胆子的根、叶、茎和果实部位分别分离得到43、19、15和8株内生细菌，其中分离自根部的YGB-11菌株对柑桔绿霉病菌、香蕉煤纹病菌等7种植物病原菌的抑菌率均超过80.00%，表现出较好的抗菌特性，结合形态特征、生理生化特征、16S rDNA序列分析和Biolog系统鉴定，确定YGB-11菌株为解淀粉芽孢杆菌（Bacillus amyloliquefacien）。【结论】内生细菌YGB-11菌株具有抑菌谱广、抑菌活性较高等特点，具有良好的应用前景。
【목적】종약용식물아담자중분리대식물병원진균구유길항활성적내생세균，위신형생물살균제적개발제공의거。【방법】채용조직균장법대아담자내생세균진행분리，통과리체화활체조직억균시험사선활성균주；근거형태특정、생리생화측정、16S rDNA서렬분석화Biolog계통대사선획득적고활성균주진행감정。【결과】종아담자적근、협、경화과실부위분별분리득도43、19、15화8주내생세균，기중분리자근부적YGB-11균주대감길록매병균、향초매문병균등7충식물병원균적억균솔균초과80.00%，표현출교호적항균특성，결합형태특정、생리생화특정、16S rDNA서렬분석화Biolog계통감정，학정YGB-11균주위해정분아포간균（Bacillus amyloliquefacien）。【결론】내생세균YGB-11균주구유억균보엄、억균활성교고등특점，구유량호적응용전경。
[Objective]Endophytic bacteria with antagonistic activity were isolated from medicinal plant Brucea javanica L. Merr. to provide references for developing new biological fungicides. [Method]The endophytic bacteria were isolated by tis-sue homogenization and the strains with antifungal activity were screened by in vitro and in vivo antifungal tests. The strains with stronger inhibitory activity was identified based on morphology, physiological and biochemical characteristics, 16S rDNA sequence analysis and Biolog test. [Result]There were 43, 19, 15 and 8 isolates obtained from root, leaf, stem and fruit of Brucea javanica, respectively. Especially, the strain YGB-11 isolated from root showed good antibiosis characteristics with antibacterial rate over 80.00%to Penicillium digitatum (pers.ex Fr.)Sacc of Citrus green mold and Helminthosporium torulosum (Syd.) Ashby of banana helminthosporium leaf spot. Based on morphological, physiological and biochemical characteristics, 16S rDNA sequence analysis and Biolog test, the strain YGB-11 was identified as Bacillus amyloliquefacien. [Conclusion]With wide antimicrobial spectrum and high antifungal activities, YGB-11 has promising prospects.