CAJ | 학술논문

【目的】探索马铃薯新品种桂农薯1号组培快繁技术，为实现工厂化生产脱毒种薯提供技术支撑。【方法】采用茎尖培养脱毒法，以马铃薯顶芽为外植体，按不同灭菌时间进行单因子（3%NaClO和0.1%HgCl2）和双因子（75%酒精和0.1%HgCl2）消毒处理，再剥取0.3~0.7 mm茎尖接种到分别添加了不同浓度GA3、6-BA及6-BA和NAA的诱导培养基上诱导成苗。将诱导出的苗进行单腋芽切段快繁，并接种到添加有6-BA和PP333的单因子及双因子组合MS培养基中进行无菌苗继代增殖。【结果】75%酒精＋0.1%HgCl2双因子灭菌较单因子3%NaClO和0.1%HgCl2效果好，污染率降至21.67%；茎尖诱导培养中，GA3和6-BA均能诱导茎尖萌芽，诱导趋势均先增后减，而以1.5 mg/L 6-BA和0.2 mg/L NAA配合，诱导率达53.67%；增殖壮苗阶段，0.1 mg/L PP333和2.5 mg/L 6-BA配合，增殖倍数达6.59倍，且苗长势好，茎粗壮、叶色正常。【结论】桂农薯1号外植体消毒最佳灭菌处理为：75%酒精2 min+0.1%HgCl211 min；最佳茎尖诱导配方为：MS+1.5 mg/L 6-BA+0.2 mg/L NAA；适合继代增殖壮苗培养基为：MS+2.5 mg/L 6-BA+0.1 mg/L PP333。
【목적】탐색마령서신품충계농서1호조배쾌번기술，위실현공엄화생산탈독충서제공기술지탱。【방법】채용경첨배양탈독법，이마령서정아위외식체，안불동멸균시간진행단인자（3%NaClO화0.1%HgCl2）화쌍인자（75%주정화0.1%HgCl2）소독처리，재박취0.3~0.7 mm경첨접충도분별첨가료불동농도GA3、6-BA급6-BA화NAA적유도배양기상유도성묘。장유도출적묘진행단액아절단쾌번，병접충도첨가유6-BA화PP333적단인자급쌍인자조합MS배양기중진행무균묘계대증식。【결과】75%주정＋0.1%HgCl2쌍인자멸균교단인자3%NaClO화0.1%HgCl2효과호，오염솔강지21.67%；경첨유도배양중，GA3화6-BA균능유도경첨맹아，유도추세균선증후감，이이1.5 mg/L 6-BA화0.2 mg/L NAA배합，유도솔체53.67%；증식장묘계단，0.1 mg/L PP333화2.5 mg/L 6-BA배합，증식배수체6.59배，차묘장세호，경조장、협색정상。【결론】계농서1호외식체소독최가멸균처리위：75%주정2 min+0.1%HgCl211 min；최가경첨유도배방위：MS+1.5 mg/L 6-BA+0.2 mg/L NAA；괄합계대증식장묘배양기위：MS+2.5 mg/L 6-BA+0.1 mg/L PP333。
[Objective]The tissue culture and rapid propagation of a new potato variety Guinongshu 1 were studied to pro-vide technical support for industrializing production of virus-free potato seeds.[Method]The stem tip detoxification method was adopted and stem tip was used as explants. The single factor (3% NaClO and 0.1% HgCl2) and two factors (75% alcohol and 0.1%HgCl2) disinfection treatments were conducted at different sterilization times. Stem tip with the size of 0.3-0.7 mm were induced into seedlings in induction medium with different concentrations of GA3, 6-BA and combinations of 6-BA and NAA. The induced single lateral bud was cut and inoculated in MS culture medium of single factor and two factors combina-tion added with 6-BA and PP33 to proliferate. [Result]The sterilization effect of two factors of 75%alcohol+0.1%HgCl2 was better than single factor of 3%NaClO or 0.1%HgCl2, and the pollution rate declined to 21.67%. Both GA3 and 6-BA could in-duce germination of stem tip with an induced trend of increase-decrease. The induction rate was as high as 53.67%for combi-nation of 1.5 mg/L 6-BA and 0.2 mg/L NAA. At multiplication stage, the multiplication rate of combination treatment adding 0.1 mg/L PP333 and 2.5 mg/L 6-BA was as high as 6.59 times and the seedlings grew better with strong stems and leaves, and normal color. [Conclusion]For Guinongshu 1 explants, the best sterilization treatment was 75%alcohol 2 min+0.1%HgCl2 11 min and the best stem tip induction formula was MS+1.5 mg/L 6-BA+0.2 mg/L NAA. The best multiplication medium was MS+2.5 mg/L 6-BA+0.1 mg/L PP333.