CAJ | 학술논문

目的：评价ApoE基因在Z：ZCLA长爪沙鼠封闭群中的遗传多样性。方法利用PCR-SSCP技术对前期已筛选到的三个SNP （ single nucleotide polymorphism ）位点在普通环境和生物净化后、屏障环境饲养的两个封闭群共444只动物中进行了ApoE等位基因的基因频率，基因型频率，杂合度、多态信息量等参数进行了检测与计算。结果检测结果表明97、781和1774三个SNP位点平均等位基因为2个，遗传方式基本符合孟德尔定律；期望杂合度分别是0.063、0.501、0.499，全群平均为0.354；PIC分别是0.061、0375、0.374，全群平均0.270。结论ApoE基因频率和基因型分布可能与长爪沙鼠的长期封闭和选种方式造成一定程度的遗传漂变相关。
목적：평개ApoE기인재Z：ZCLA장조사서봉폐군중적유전다양성。방법이용PCR-SSCP기술대전기이사선도적삼개SNP （ single nucleotide polymorphism ）위점재보통배경화생물정화후、병장배경사양적량개봉폐군공444지동물중진행료ApoE등위기인적기인빈솔，기인형빈솔，잡합도、다태신식량등삼수진행료검측여계산。결과검측결과표명97、781화1774삼개SNP위점평균등위기인위2개，유전방식기본부합맹덕이정률；기망잡합도분별시0.063、0.501、0.499，전군평균위0.354；PIC분별시0.061、0375、0.374，전군평균0.270。결론ApoE기인빈솔화기인형분포가능여장조사서적장기봉폐화선충방식조성일정정도적유전표변상관。
Objective To evaluate the genetic diversity of ApoE gene in Z:ZCLA gerbil closed colony .Methods PCR-SSCP technique was employed to calculate the gene frequency , genotype frequency , heterozygosity , polymorphism information content of three SNPs of ApoE gene , which were reported in our previous work , in a total of 444 ordinary and clean Mongolian gerbils .Results The average allele of 3 SNP site 97, 981 and 1774 was 2.The inheritance pattern was in accordance with Mendel′s law.Expected heterozygosities were 0.063, 0.501, and 0.499, and the average expected heterozygosity of whole group was 0.354.PIC were 0.061, 0375, and 0.374, with an average PIC of 0.270.Conclusion The ApoE gene frequency and genotype distribution in Mongolian gerbils may be to a certain degree associated with the genetic drift caused by long-term closed and selected breeding .