CAJ | 학술논문

目的：研究饮食诱发的高脂血症长爪沙鼠模型的关键基因及代谢性炎症通路调控网络。方法利用RNA-Seq测序技术及生物信息学技术对正常组与模型组两个RNA池进行测序和分析。结果 De novo拼接后获得有效长爪沙鼠基因47522个（即≥100 bp的unigene），大小26.9 Mb，与GenBank中现有的基因比对结果表明，约82.53％的序列与基因组上的外显子序列同源；诱发高脂血症动物与正常动物之间共获得21125个差异基因，其中16087个下调，5038个上调，模型组相对于正常组存在显著的下调关系（ P＜0.01）。其中与长爪沙鼠高脂血症代谢性炎症密切相关的通路有8个（P＜0.01），这8个通路所表达的基因中有15个与免疫和炎症相关的基因显著下调（ P＜0.01）。结论 RNA-Seq测序和生物信息技术可作为研究高脂血症动物模型的整体基因转录的工具，筛选出的关键基因和通路可作为研究的候选基因或操作靶点。
목적：연구음식유발적고지혈증장조사서모형적관건기인급대사성염증통로조공망락。방법이용RNA-Seq측서기술급생물신식학기술대정상조여모형조량개RNA지진행측서화분석。결과 De novo병접후획득유효장조사서기인47522개（즉≥100 bp적unigene），대소26.9 Mb，여GenBank중현유적기인비대결과표명，약82.53％적서렬여기인조상적외현자서렬동원；유발고지혈증동물여정상동물지간공획득21125개차이기인，기중16087개하조，5038개상조，모형조상대우정상조존재현저적하조관계（ P＜0.01）。기중여장조사서고지혈증대사성염증밀절상관적통로유8개（P＜0.01），저8개통로소표체적기인중유15개여면역화염증상관적기인현저하조（ P＜0.01）。결론 RNA-Seq측서화생물신식기술가작위연구고지혈증동물모형적정체기인전록적공구，사선출적관건기인화통로가작위연구적후선기인혹조작파점。
Objective To understand some key genes and metabolic/inflammatory pathways triggered by high fat/high cholesterol ( HF/HC ) diet-induced hyperlipidemia in Mongolian gerbil .Methods Sixty 90-day-old Mongolian gerbils (including both sexes) were used in this study.The gerbils were divided into normal group (n=30, control diets) and model group (n=30, Hf/HC diets).Hyperlipidemia was induced for 4 weeks.To extract mRNA for transcriptome by RNA-Seq deep sequencing and bioinformatics .Results The results showed that 47 522 de novo gerbil genes (≥100 bp unigene ) were obtained , with a total of 26.9 Mb, and about 82.53% sequence was homologous with the exon in the common genome bases .There were 21 125 differential genes between the hyperlipidemia gerbil and normal gerbil groups , including 16 087 down-regulated and 5038 up-regulated genes .There was a significant trend of downregulation compared with the normal group ( P <0.01 ) .There were eight pathways significantly related to the metabolic inflammation in hyperlipidemia ( P<0.01) , and among the expressed genes of these pathways , 15 immune-and inflammation-related genes were significantly down-regulated ( P<0.01 ) .Conclusions RNA-Seq and bioinformatics techniques can be used as a powerful tool for overall gene-transcription in hyperlipidemic gerbil models .Some key genes and pathways screened in this study may become candidate genes or operating targets in the future research .